Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MOLT-4 (Human lymphoblastic leukemia) cells labeling IRF1 with ab186384 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing nuclear and cytoplasmic staining on MOLT-4 cells.The nuclear counterstain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:--ve control 1 - ab186384 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia from peripheral blood) cells labeling IRF1 with ab186384 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing cytoplasmic and weakly nuclear staining on Jurkat cells.The nuclear counterstain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:--ve control 1 - ab186384 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling IRF1 with ab186384 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing cytoplasmic and weakly nuclear staining on NIH/3T3 cells.The nuclear counterstain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:--ve control 1 - ab186384 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cells) cells labeling IRF1 with ab186384 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing cytoplasmic and nuclear staining on C6 cells.The nuclear counterstain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:--ve control 1 - ab186384 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
![All lanes : Anti-IRF1 antibody [EPR18301] (ab186384) at 1/10000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 2 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysateLane 3 : HeLa whole cell lysates treated with 10 ng/ml IFN-gamma for 24 hoursLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/9947_ab186384-245913-1863849.JPG)
All lanes : Anti-IRF1 antibody [EPR18301] (ab186384) at 1/10000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 2 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysateLane 3 : HeLa whole cell lysates treated with 10 ng/ml IFN-gamma for 24 hoursLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
![Anti-IRF1 antibody [EPR18301] (ab186384) at 1/1000 dilution + MOLT-4 (Human lymphoblastic leukemia) whole cell lysate 20ugSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/9948_ab186384-245912-1863848.JPG)
Anti-IRF1 antibody [EPR18301] (ab186384) at 1/1000 dilution + MOLT-4 (Human lymphoblastic leukemia) whole cell lysate 20ugSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
![All lanes : Anti-IRF1 antibody [EPR18301] (ab186384) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse spleen lysateLane 4 : Rat brain lysateLane 5 : Rat heart lysateLane 6 : C6 (rat glioma tumor) whole cell lysateLane 7 : RAW 264.7 (mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 8 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysateLane 9 : NIH/3T3 (mouse embryo fibroblast) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/9949_ab186384-245911-1863847.JPG)
All lanes : Anti-IRF1 antibody [EPR18301] (ab186384) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse spleen lysateLane 4 : Rat brain lysateLane 5 : Rat heart lysateLane 6 : C6 (rat glioma tumor) whole cell lysateLane 7 : RAW 264.7 (mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 8 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysateLane 9 : NIH/3T3 (mouse embryo fibroblast) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells fromperipheral blood) cells labeling IRF1 with ab186384 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling IRF1 with ab186384 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Nuclear and cytoplasmic staining on epithelial cells of normal Human colon is observed.Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling IRF1 with ab186384 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Nuclear and weak cytoplasmic staining on tumor cells of gastric adenocarcinoma is observed.Counter stained with Hematoxylin.Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
IRF1 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab186384 at 1/80 dilution.Lane 1: Input Jurkat whole cell extract (10µg). Lane 2: Jurkat whole cell lysate following immunoprecipitation with ab186384.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab186384 in Jurkat whole cell lysate.Western blot was performed from the immunoprecipitate using ab186384 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as secondary antibody at 1/1500 dilution.Blocking and dilution buffer and concentration: 5% NFDM/TBST. 30 second exposure.