Anti-IRF5 antibody [10T1]
| Name | Anti-IRF5 antibody [10T1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab33478 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 10T1 |
| Applications | ICC/IF ICC/IF ELISA IP WB FC IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant human IRF-5 (176-240 aa, isoform D) purified from E |
| Description | Mouse Monoclonal |
| Gene | IRF5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Immunofluorescence/Immunocytochemistry analysis of HEK293 cells (105-106) stained with Mouse monoclonal [10T1] to IRF5 (ab33478 1/100-1/200).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10062_ab33478_2.gif)
Immunofluorescence/Immunocytochemistry analysis of HEK293 cells (105-106) stained with Mouse monoclonal [10T1] to IRF5 (ab33478 1/100-1/200).
![All lanes : Anti-IRF5 antibody [10T1] (ab33478) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : THP-1 cell lysateLane 3 : A20 cell lysateLane 4 : NIH 3T3 cell lysateSecondarygoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10063_ab33478wb.jpg)
All lanes : Anti-IRF5 antibody [10T1] (ab33478) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : THP-1 cell lysateLane 3 : A20 cell lysateLane 4 : NIH 3T3 cell lysateSecondarygoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique
![Overlay histogram showing THP1 cells stained with ab33478 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33478, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10064_IRF5-Primary-antibodies-ab33478-10.jpg)
Overlay histogram showing THP1 cells stained with ab33478 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33478, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ab33478 at 1/50 dilution staining IRF5 in Human normal colon mucosa tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The section was incubated with the primary antibody for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
ab33478 at 1/50 dilution staining IRF5 in Human colon adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The section was incubated with the primary antibody for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Product References
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NOD2, RIP2 and IRF5 play a critical role in the type I interferon response to - NOD2, RIP2 and IRF5 play a critical role in the type I interferon response to
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