IRF5

Gene Symbol	IRF5
Entrez Gene	3663
Alt Symbol	SLEB10
Species	Human
Gene Type	protein-coding
Description	interferon regulatory factor 5
Other Description	-
Swissprots	E7EQ16 Q6RCM8 Q1A7B4 Q64GB1 E7EW54 Q64GB2 Q9BQF0 A8DUA8 A4D1J8 Q64GA9 Q13568 A8DUA9
Accessions	ABK41901 ABL96293 ABL96294 EAL24107 EAL24108 EAW83703 EAW83704 EAW83705 EAW83706 EAW83707 Q13568 AK293654 BAH11558 AK297083 BAG59600 AL713733 AY504946 AAR90325 AY504947 AAR90326 AY693665 AAU12877 AY693666 AAU12878 AY693667 AAU12879 AY693668 AAU12880 AY693669 AAU12881 BC004139 AAH04139 BC004201 AAH04201 BU540632 BU950658 CA427676 CA444548 DC427600 DQ277633 ABB88960 DQ277634 ABB88961 DQ893637 ABM84563 DQ995491 DQ995492 DQ995493 DQ995494 EU176684 ABW03485 EU258895 ABX54712 EU258896 ABX54713 EU258897 ABX54714 EU258898 ABX54715 JQ950681 AFN70752 JQ950682 AFN70753 JQ950683 AFN70754 JQ950684 AFN70755 JQ950685 AFN70756 U51127 AAA96056 XM_005250317 XP_005250374 XM_006715974 XP_006716037 XM_011516158 XP_011514460 XM_011516159 XP_011514461 XM_011516160 XP_011514462 XM_011516161 XP_011514463 XM_011516162 XP_011514464 XM_011516163 XP_011514465 XM_011516164 XP_011514466 NM_001098627 NP_001092097 NM_001098629 NP_001092099 NM_001098630 NP_001092100 NM_001242452 NP_001229381 NM_032643 NP_116032
Function	Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. {ECO:0000269\|PubMed:11303025, ECO:0000269\|PubMed:15695821}.
Subcellular Location	Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm.
Top Pathways	Toll-like receptor signaling pathway

IRF-5 (E1N9G) Rabbit mAb - 13496 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	E1N9G

Western blot analysis of extracts from various cell lines using IRF-5 (E1N9G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IRF-5 Antibody (Rodent Specific) - 4950 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from Raw264.7 cells, untreated or LPS-treated (1 µg/ml for 6 h), M12.4.1 cells and A20 cells, using IRF-5 Antibody (Rodent Specific).

IRF-5 Antibody - 3257 from Cell Signaling Technology

Host	Rabbit
Clonality	Polyclonal

IRF-5 (C-6) - sc-390364 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

IRF-5 (C-6): sc-390364. Western blot analysis of IRF-5 expression in SR (A) and Ramos (B) whole cell lysates.

IRF-5 (D-10) - sc-390365 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

IRF-5 (D-10): sc-390365. Western blot analysis of IRF-5 expression in SR (A) and Ramos (B) whole cell lysates.

IRF-5 (10T1) - sc-56714 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Mouse
Clonality	Monoclonal

IRF-5 (10T1): sc-56714. Western blot analysis of IRF-5 expression in Ramos (A), THP-1 (B) and A20 (C) whole cell lysates.

IRF-5 (H-56) - sc-98651 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

IRF-5 (H-56): sc-98651. Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in white pulp and cells in red pulp.

Anti-IRF5 antibody - ab175317 from Abcam

Prices	$379.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175317. Blue DAPI for nuclear staining.

Anti-IRF5 antibody - ab140593 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-IRF5 antibody (ab140593) at 5 µg/ml + Recombinant protein at 0.2 µgSecondaryGoat Anti-mouse HRP at 1/5000 dilution

Anti-IRF5 antibody [10T1] - ab33478 from Abcam

Prices	$392.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	10T1

Immunofluorescence/Immunocytochemistry analysis of HEK293 cells (105-106) stained with Mouse monoclonal [10T1] to IRF5 (ab33478 1/100-1/200).

Anti-IRF5 antibody [EPR17067] - ab181553 from Abcam

Prices	$347.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR17067

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cells labeling IRF5 with ab181553 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining on RAW 264.7 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab181553 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Anti-IRF5 antibody [EPR6094] - ab124792 from Abcam

Prices	$394.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR6094

All lanes : Anti-IRF5 antibody [EPR6094] (ab124792) at 1/1000 dilutionLane 1 : U937 cell lysatesLane 2 : Ramos cell lysatesLane 3 : THP-1 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-IRF5 antibody [EPR6094] (Alexa Fluor® 488) - ab193245 from Abcam

Prices	$431.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR6094

ab193245 staining IRF5 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab193245 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-IRF5 antibody [EPR6094] (Alexa Fluor® 647) - ab192983 from Abcam

Prices	$425.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR6094

ab192983 staining IRF5 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192983 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-IRF5 Antibody (Ser172) - LS-C36968 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IRF5 Antibody (C-Terminus) - LS-C36969 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IRF5 Antibody (aa121-170) - LS-C30187 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-IRF5 Antibody (aa176-240) - LS-C45031 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-IRF5 Antibody - LS-C70827 from LifeSpan Bioscience

Prices	$605.00
Sizes	100 µg
Host	Sheep
Clonality	Polyclonal

Anti-IRF5 Antibody (N-Terminus) - LS-C31672 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-IRF5 Antibody (aa503-514) - LS-C20170 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Staining (2 ug/ml) of A549 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-IRF5 Antibody (aa175-225) - LS-C292258 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IRF5 Antibody (aa275-325) - LS-C292259 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-IRF5 Antibody - LS-C310778 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-IRF5 Antibody (clone 10T1) - LS-C93755 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	10T1

The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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