Anti-IRF7 antibody
| Name | Anti-IRF7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab62505 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human IRF7 |
| Blocking Peptide | Human IRF7 peptide |
| Description | Rabbit Polyclonal |
| Gene | IRF7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab62505 stained HepG2 cells (ab7900). The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62505, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of IRF7 in Mouse Spleen cells using ab62505 at 20 ug/ml.
Lane 1 : Anti-IRF7 antibody (ab62505) at 0.5 µg/mlLane 2 : Anti-IRF7 antibody (ab62505) at 1 µg/mlLane 3 : Anti-IRF7 antibody (ab62505) at 2 µg/mlLane 1 : 293 whole cell lysateLane 2 : 293 whole cell lysateLane 3 : 293 whole cell lysateLysates/proteins at 15 µg per lane.
Immunohistochemistry of IRF7 in paraffin embedded mouse spleen tissue section with ab62505 antibody at 5 µg/ml.
![IRF7 was immunoprecipitated using 0.5mg Jurkat whole cell extract (ab7899), 5µg of Rabbit polyclonal to IRF7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab62505.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 49kDa: IRF7; non specific - 40kDa: We are unsure as to the identity of this extra band.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10114_IRF7-Primary-antibodies-ab62505-4.jpg)
IRF7 was immunoprecipitated using 0.5mg Jurkat whole cell extract (ab7899), 5µg of Rabbit polyclonal to IRF7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab62505.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 49kDa: IRF7; non specific - 40kDa: We are unsure as to the identity of this extra band.
Product References
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Schmeisser H, Mejido J, Balinsky CA, Morrow AN, Clark CR, Zhao T, Zoon KC. J Virol. 2010 Oct;84(20):10671-80.