![All lanes : Anti-IRF8 antibody [mAbcam61750] (ab61750) at 10 µg/mlLane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10138_IRF8-Primary-antibodies-ab61750-1.jpg)
All lanes : Anti-IRF8 antibody [mAbcam61750] (ab61750) at 10 µg/mlLane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing K562 cells stained with ab61750 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61750, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10139_IRF8-Primary-antibodies-ab61750-3.jpg)
Overlay histogram showing K562 cells stained with ab61750 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61750, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.