Anti-IRGM antibody
| Name | Anti-IRGM antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab69494 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human |
| Antigen | A 17 amino acid peptide near the amino terminus of human IRGM |
| Description | Rabbit Polyclonal |
| Gene | IRGM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab69494 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69494, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Irgm1 (LRG-47), a regulator of cell-autonomous immunity, does not localize to - Irgm1 (LRG-47), a regulator of cell-autonomous immunity, does not localize to
Springer HM, Schramm M, Taylor GA, Howard JC. J Immunol. 2013 Aug 15;191(4):1765-74.
Human IRGM regulates autophagy and cell-autonomous immunity functions through - Human IRGM regulates autophagy and cell-autonomous immunity functions through
Singh SB, Ornatowski W, Vergne I, Naylor J, Delgado M, Roberts E, Ponpuak M, Master S, Pilli M, White E, Komatsu M, Deretic V. Nat Cell Biol. 2010 Dec;12(12):1154-65.