Anti-IRS4 antibody [EP907Y]
| Name | Anti-IRS4 antibody [EP907Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52622 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP907Y |
| Applications | IHC-P WB IP FC |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to residues from the C terminus of human IRS4 |
| Description | Rabbit Monoclonal |
| Gene | IRS4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-IRS4 antibody [EP907Y] (ab52622) at 1/5000 dilution + Hek293 cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10229_ab52622_WB_1.jpg)
Anti-IRS4 antibody [EP907Y] (ab52622) at 1/5000 dilution + Hek293 cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution
ab52622, at a 1/50 dilution staining Human IRS4 in kidney using Immunohistochemistry, Paraffin embedded tissue.
Overlay histogram showing HEK293 cells stained with ab52622 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52622, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes - Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes
Homma Y, Kanno S, Sasaki K, Nishita M, Yasui A, Asano T, Ohashi K, Mizuno K. J Biol Chem. 2014 Sep 19;289(38):26302-13.
A novel transcript is up-regulated by fasting in the hypothalamus and enhances - A novel transcript is up-regulated by fasting in the hypothalamus and enhances
Chai B, Li JY, Fritze D, Zhang W, Xia Z, Mulholland MW. J Neuroendocrinol. 2013 Mar;25(3):292-301.
The t(X;6) in subungual exostosis results in transcriptional deregulation of the - The t(X;6) in subungual exostosis results in transcriptional deregulation of the
Mertens F, Moller E, Mandahl N, Picci P, Perez-Atayde AR, Samson I, Sciot R, Debiec-Rychter M. Int J Cancer. 2011 Jan 15;128(2):487-91.
The t(X;7)(q22;q34) in paediatric T-cell acute lymphoblastic leukaemia results in - The t(X;7)(q22;q34) in paediatric T-cell acute lymphoblastic leukaemia results in
Karrman K, Kjeldsen E, Lassen C, Isaksson M, Davidsson J, Andersson A, Hasle H, Fioretos T, Johansson B. Br J Haematol. 2009 Feb;144(4):546-51.