Anti-Islet 1 antibody [1H9]
| Name | Anti-Islet 1 antibody [1H9] |
|---|---|
| Supplier | Abcam |
| Catalog | ab86472 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1H9 |
| Applications | FC WB ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant fragment corresponding to Human Islet 1 aa 150-349 |
| Description | Mouse Monoclonal |
| Gene | ISL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Islet 1 antibody [1H9] (ab86472) at 1/500 dilution + HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10308_MPS1-Primary-antibodies-ab86472-1.jpg)
Anti-Islet 1 antibody [1H9] (ab86472) at 1/500 dilution + HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc
Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length Islet 1-hIgGFc using ab86472 at 1/150 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
ab86472 at a 1/200 dilution staining Islet 1 in human lung cancer tissue. DAB staining shows nuclear localisation.
ab86472 at a 1/200 dilution staining Islet 1 in human cervical carcinoma tissue. DAB staining shows nuclear localization.
![Overlay histogram showing SH-SY5Y cells stained with ab86472 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86472, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10312_ab86472-169470-Islet1Primaryantibodiesab864725.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab86472 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86472, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
ISL-1 is overexpressed in non-Hodgkin lymphoma and promotes lymphoma cell - ISL-1 is overexpressed in non-Hodgkin lymphoma and promotes lymphoma cell
Zhang Q, Yang Z, Jia Z, Liu C, Guo C, Lu H, Chen P, Ma K, Wang W, Zhou C. Mol Cancer. 2014 Jul 29;13:181.
ISL1 protein transduction promotes cardiomyocyte differentiation from human - ISL1 protein transduction promotes cardiomyocyte differentiation from human
Fonoudi H, Yeganeh M, Fattahi F, Ghazizadeh Z, Rassouli H, Alikhani M, Mojarad BA, Baharvand H, Salekdeh GH, Aghdami N. PLoS One. 2013;8(1):e55577.
ISL1 expression is not restricted to pancreatic well-differentiated - ISL1 expression is not restricted to pancreatic well-differentiated
Agaimy A, Erlenbach-Wunsch K, Konukiewitz B, Schmitt AM, Rieker RJ, Vieth M, Kiesewetter F, Hartmann A, Zamboni G, Perren A, Kloppel G. Mod Pathol. 2013 Jul;26(7):995-1003.
Brief demethylation step allows the conversion of adult human skin fibroblasts - Brief demethylation step allows the conversion of adult human skin fibroblasts
Pennarossa G, Maffei S, Campagnol M, Tarantini L, Gandolfi F, Brevini TA. Proc Natl Acad Sci U S A. 2013 May 28;110(22):8948-53. doi: