IF/ICC image of ab109517 stained U-87 MG cells.
Jurkat whole cell lysate was for this immunoprecipitation experiment.
All lanes : Anti-Islet 1 antibody [EP4182] (ab109517) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.
IHC-Fr image of anti-Islet 1 staining with ab109517 on tissue sections from chicken hindbrain. The sections were blocked with 3% BSA for 1 hour at 4°C, before incubation with ab109517 (1/1000 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor 568 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.See Abreview