All lanes : Anti-Islet 1 antibody [EPR10362] (ab178400) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : Jurkat cell lysateLane 4 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human gastric neuroendocrine carcinoma tissue labeling Islet 1 with ab178400 at a 1/250 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human small cell lung carcinoma tissue labeling Islet 1 with ab178400 at a 1/250 dilution.
Immunofluorescence analysis of SH-SY5Y cells labeling Islet 1 with ab178400 at a 1/250 dilution.
Western blot analysis on Immunoprecipitation pellet from either 1) SH-SY5Y cell lysate, or 2) 1xPBS (negative control); showing Islet 1, using ab178400 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.