All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilutionLane 1 : Human fetal skin lysateLane 2 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-ITGB6 antibody [EPR15740] (ab187155) at 1/10000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Lysate from 293 cells was immunoprecipitated with ab187155 at a 1/50 dilution. For the subsequent blot, ab187155 used at a 1/10000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.