Anti-ITK antibody [Y401]
| Name | Anti-ITK antibody [Y401] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32039 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y401 |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to residues in the N terminus of human Emtk/ITK/Tsk |
| Description | Rabbit Monoclonal |
| Gene | ITK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-ITK antibody [Y401] (ab32039) at 1/5000 dilution + Jurkat cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10538_ab32039wb.gif)
Anti-ITK antibody [Y401] (ab32039) at 1/5000 dilution + Jurkat cell lysate
Ab32039 at a 1/250 dilution, staining ITK in paraffin embedded human lymph node tissue by Immunohistochemistry.
Overlay histogram showing Jurkat cells stained with ab32039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32039, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.