ITK

Gene Symbol ITK
Entrez Gene 3702
Alt Symbol EMT, LPFS1, LYK, PSCTK2
Species Human
Gene Type protein-coding
Description IL2-inducible T-cell kinase
Other Description IL-2-inducible T cell kinase|IL-2-inducible T-cell kinase|T-cell-specific kinase|homolog of mouse T-cell itk/tsk|interleukin-2-inducible T cell kinase|interleukin-2-inducible T-cell kinase|kinase EMT|tyrosine-protein kinase ITK/TSK|tyrosine-protein kinase LYK
Swissprots B2R752 Q32ML7 Q08881
Accessions EAW61608 Q08881 AB209622 BAD92859 AK303609 BAG64622 AK311654 AK312846 BAG35699 BC094701 BC109077 AAI09078 BC109078 AAI09079 BX538196 CAD98063 D13720 BAA02873 NM_005546 NP_005537
Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ulti
Subcellular Location Cytoplasm {ECO:0000269|PubMed:17060314}. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
Tissue Specificity T-cell lines and natural killer cell lines.
Top Pathways T cell receptor signaling pathway, Leukocyte transendothelial migration, Chemokine signaling pathway

Itk (2F12) Mouse mAb - 2380 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 2F12
Western blotting analysis of various cell extracts using Itk (2F12) Mouse mAb.

Emt (G-11) - sc-514176 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Emt (G-11): sc-514176. Western blot analysis of Emt expression in CTLL-2 (A), TK-1 (B) and K-562 (C) whole cell lysates. Note lack of reactivity with human Emt in Lane C.

Emt (C-20) - sc-1103 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Emt (C-20): sc-1103. Western blot analysis of Emt expression in HuT 78 (A) and CTLL-2 (B) whole cell lysates.

Emt (2F12) - sc-23902 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Emt (2F12): sc-23902. Western blot analysis of Emt expression in Jurkat (A), Hut 78 (B), CTLL-2 (C) and TK-1 (D) whole cell lysates.

Emt (M-109) - sc-1697 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Emt (M-109): sc-1697. Western blot analysis of Emt expression in human PBL whole cell lysate.

Emt (M-20) - sc-1104 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Emt (M-20): sc-1104. Western blot analysis of Emt expression in HuT 78 whole cell lysate.

Emt (0.T.74) - sc-71040 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Emt (3F227) - sc-71041 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Emt (SPM409) - sc-56383 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-ITK antibody - ab32113 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ITK antibody (ab32113) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : A431 cell lysate

Anti-ITK antibody - ab37826 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab37826 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37826, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ITK antibody - ab9707 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab9707 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Anti-ITK antibody - ab189388 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human spleen tissue sections labeling ITK using ab189388 at 3.75 µg/ml.

Anti-ITK antibody [5G6] - ab119885 from Abcam

Host Mouse
Clonality Monoclonal
Clone 5G6
Anti-ITK antibody [5G6] (ab119885) at 1/500 dilution + ITK recombinant protein

Anti-ITK antibody [Y401] - ab32039 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y401
Anti-ITK antibody [Y401] (ab32039) at 1/5000 dilution + Jurkat cell lysate

Anti-ITK antibody [Y402] - ab32507 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y402
Overlay histogram showing Jurkat cells stained with ab32507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ITK Antibody (aa1-270) - LS-C16091 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ITK Antibody (C-Terminus) - LS-C24918 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ITK Antibody - LS-C26703 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-ITK / EMT Antibody (aa25-54, AP) - LS-C260545 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ITK / EMT Antibody (aa25-54, Biotin) - LS-C260547 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ITK / EMT Antibody (aa25-54, APC) - LS-C260546 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ITK / EMT Antibody (aa25-54, FITC) - LS-C260548 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ITK / EMT Antibody (aa25-54, Azide-free, HRP) - LS-C260549 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ITK / EMT Antibody (aa25-54, PE) - LS-C260550 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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