Anti-ITK antibody [Y402]
| Name | Anti-ITK antibody [Y402] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32507 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y402 |
| Applications | WB FC IP |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to residues near the C-term of human ITK |
| Description | Rabbit Monoclonal |
| Gene | ITK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing Jurkat cells stained with ab32507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-ITK antibody [Y402] (ab32507) at 1/1000 dilution + Jurkat cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10542_ab32507wb.gif)
Anti-ITK antibody [Y402] (ab32507) at 1/1000 dilution + Jurkat cell lysate