Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175222 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized HeLa cells labeling ITPK1 with ab175222 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITPK1 with ab175222 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ITPK1 with ab175222 at 1/50 dilution.
All lanes : Anti-ITPK1 antibody [EPR10631] (ab175222) at 1/10000 dilutionLane 1 : Fetal brain lysateLane 2 : Fetal liver lysateLane 3 : HepG2 cell lysateLane 4 : Hela cell lysateLysates/proteins at 10 µg per lane.