Anti-JAB1 antibody
| Name | Anti-JAB1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12323 |
| Prices | $380.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Dog, Turkey, Pig, Xenopus, Chimpanzee, Zebrafish, Monkey, Orangutan, Xenopus |
| Antigen | Immunogen was a synthetic peptide, which represented a c-terminal domain of human JAB1 (LocusLink ID 10987) |
| Description | Rabbit Polyclonal |
| Gene | COPS5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling JAB1 with ab12323 at 1/500 (2µg/ml). Detection: DAB.
All lanes : Anti-JAB1 antibody (ab12323) at 1/2500 dilutionLane 1 : HEK293 whole cell lysateLane 2 : HCT116 whole cell lysateLane 3 : U2OS whole cell lysateLane 4 : Mouse embryonic fibroblast whole cell lysateLysates/proteins at 35 µg per lane.SecondaryHRP-conjugated Goat anti-rabbit polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab12323 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12323, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Regulation of cullin RING E3 ubiquitin ligases by CAND1 in vivo. - Regulation of cullin RING E3 ubiquitin ligases by CAND1 in vivo.
Chua YS, Boh BK, Ponyeam W, Hagen T. PLoS One. 2011 Jan 13;6(1):e16071.
Biochemical and cellular effects of inhibiting Nedd8 conjugation. - Biochemical and cellular effects of inhibiting Nedd8 conjugation.
Leck YC, Choo YY, Tan CY, Smith PG, Hagen T. Biochem Biophys Res Commun. 2010 Jul 30;398(3):588-93. doi:
Substrate-mediated regulation of cullin neddylation. - Substrate-mediated regulation of cullin neddylation.
Chew EH, Hagen T. J Biol Chem. 2007 Jun 8;282(23):17032-40. Epub 2007 Apr 17.