All lanes : Anti-Jarid2 antibody [EPR6357(2)] (ab192252) at 1/10000 dilutionLane 1 : NCCIT cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-Jarid2 antibody [EPR6357(2)] (ab192252) at 1/1000 dilution + U87-MG cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized SH-SY5Y cells labeling Jarid2 with ab192252 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue). The two negative controls are ab192252 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.