All lanes : Anti-JIP2 antibody (ab65211) at 1/500 dilutionLane 1 : Extracts from mouse brain cellsLane 2 : Extracts from mouse brain cells, plus immunising peptide
ab65211 staining the JIP2 (Red) in Zebra finch basal ganglia (striatum) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.3% Triton and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in PBS Tween) for 24 hours at 4°C. An Alexa Fluor®555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (Blue). See Abreview
ICC/IF image of ab65211 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65211, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.