Anti-JLP antibody
| Name | Anti-JLP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12331 |
| Prices | $380.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IP |
| Species Reactivities | Mouse, Rat, Human, Guinea Pig, Dog, Pig, Chimpanzee, Monkey, Opossum, Orangutan, Elephant |
| Antigen | Immunogen was a synthetic peptide, which represented a portion of human JLP encoded within exon 1 |
| Description | Rabbit Polyclonal |
| Gene | SPAG9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis (left) and testicular seminoma (right) tissues labelling JLP with ab12331 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
ICC/IF image of ab12331 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12331, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-JLP antibody (ab12331) at 0.2 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
All lanes : Anti-JLP antibody (ab12331) at 1 µg/mlLane 1 : NIH3T3 whole cell lysateLane 2 : TCMK-1 whole cell lysateLane 3 : 4T1 whole cell lysateLane 4 : CT26.WT whole cell lysateLane 5 : C6 whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique
Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded). ab12331 was used for immunoprecipitation at 3 µg/mg lysate and used for western blot at 1 µg/ml. Lanes 1 and 2 - ab12331 IP, Lane 3 - control IgG.
Product References
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Lu M, Krauss RS. Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4212-7.
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Oh JE, Bae GU, Yang YJ, Yi MJ, Lee HJ, Kim BG, Krauss RS, Kang JS. FASEB J. 2009 Jul;23(7):2088-99.
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Bae GU, Kim BG, Lee HJ, Oh JE, Lee SJ, Zhang W, Krauss RS, Kang JS. Mol Cell Biol. 2009 Aug;29(15):4130-43.
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Chen Y, Choong LY, Lin Q, Philp R, Wong CH, Ang BK, Tan YL, Loh MC, Hew CL, Shah N, Druker BJ, Chong PK, Lim YP. Mol Cell Proteomics. 2007 Dec;6(12):2072-87. Epub 2007 Sep 12.
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