Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling JLP with ab12332 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique
All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/mlLane 1 : TCKM whole cell lysate at 50 µgLane 2 : Renca whole cell lysate at 15 µgLane 3 : CT26 whole cell lysate at 50 µgdeveloped using the ECL technique
Immunoprecipitation analaysis of HeLa whole cell lysate (1 mg for IP; 20% of IP loaded). ab12332 was used for immunoprecipitation at 6 µg/mg lysate (lanes 1 and 2). Lane 3 - control IgG. For western blot ab12332 was used at 1 µg/ml.