Anti-JNK2 antibody (ab88076) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Anti-JNK2 antibody (ab88076) at 5 µg/ml + Jurkat cell lysate at 25 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
ab88076 at 10µg/ml staining JNK2 in Hela cells.
Detection limit for ab88076 is approximately 0.03ng/ml as a capture antibody.
Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF2 and JNK2 HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1/1200 and ab88076 1/50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.