All lanes : Anti-JNK3 antibody (ab87404) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 3 : Mouse Hippocampus Tissue Lysate Lane 4 : Cerebellum Mouse Tissue LysateLane 5 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate Lane 6 : Rat Hippocampus Tissue LysateLane 7 : Cerebellum Rat Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution