MAPK10

Gene Symbol MAPK10
Entrez Gene 5602
Alt Symbol JNK3, JNK3A, PRKM10, SAPK1b, p493F12, p54bSAPK
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase 10
Other Description JNK3 alpha protein kinase|MAP kinase 10|MAP kinase p49 3F12|MAPK 10|c-Jun N-terminal kinase 3|stress activated protein kinase beta|stress-activated protein kinase 1b|stress-activated protein kinase JNK3
Swissprots A6NG28 B3KQ94 Q15707 A6NFS3 Q49AP1 P53779
Accessions CAC09126 CAP12256 CAP12257 CAP12259 EAX05962 EAX05963 P53779 AF052141 AK022161 AK057723 BAG51956 AK091104 BAG52284 AK124791 BAG54096 AK225974 AK294125 BAH11677 AK307129 BC022492 BC035057 AAH35057 BC051731 AAH51731 BC065516 AAH65516 BI549234 DA192997 HG497056 U07620 AAC50101 U34819 AAC50604 U34820 AAC50605 XM_005263129 XP_005263186 XM_005263130 XP_005263187 XM_005263131 XP_005263188 XM_005263135 XP_005263192 XM_005263136 XP_005263193 XM_006714268 XP_006714331 XM_006714269 XP_006714332 XM_006714271 XP_006714334 XM_006714272 XP_006714335 XM_011532117 XP_011530419 XM_011532118 XP_011530420 XM_011532119 XP_011530421 XM_011532120 XP_011530422 XM_011532121 XP_011530423 XM_011532122 XP_011530424 XM_011532123 XP_011530425 NM_002753 NP_002744 NM_138980 NP_620446 NM_138981 NP_620447 NM_138982 NP_620448
Function Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress- activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a rol
Subcellular Location Cytoplasm {ECO:0000269|PubMed:16737965}. Membrane {ECO:0000269|PubMed:16737965}; Lipid-anchor {ECO:0000269|PubMed:16737965}. Nucleus {ECO:0000269|PubMed:16737965}. Mitochondrion {ECO:0000269|PubMed:16737965}. Note=Palmitoylation regulates MAPK10 trafficking to cytoskeleton. Recruited to the mitochondria in the presence of SARM1 (By similarity). {ECO:0000250}.
Tissue Specificity Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
Top Pathways Wnt signaling pathway, Herpes simplex infection, Dopaminergic synapse, Choline metabolism in cancer, cAMP signaling pathway

JNK3 (55A8) Rabbit mAb - 2305 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 55A8
Western blot analysis of recombinant His-tagged JNK kinases (left), or brain lysates from wild type (WT) and specific JNK knockout (-/-) mice (right), using JNK3 (55A8) Rabbit mAb (Image provided by Drs Gerardo Morfini, YiMei You and Scott Brady, University of Illinois at Chicago).

JNK3 (G-18) - sc-46014 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
JNK3 (G-18): sc-46014. Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of neuronal cells.

JNK3 (N-16) - sc-46015 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

JNK3 (10E4A4) - sc-130075 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
JNK3 (10E4A4): sc-130075. Western blot analysis of JNK3 expression in NIH/3T3 (A) and SK-N-SH (B) whole cell lysates.

JNK3 (4G6) - sc-81469 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
JNK3 (4G6): sc-81469. Western blot analysis of JNK3 expression in non-transfected 293T: sc-117752 (A), human JNK3 transfected 293T: sc-116954 (B) and HeLa (C) whole cell lysates.

Anti-JNK3 antibody - ab197050 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-JNK3 antibody (ab197050) at 1/500 dilutionLane 1 : mouse brain lysateLane 2 : mouse testis lysate

Anti-JNK3 antibody - ab70882 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-JNK3 antibody (ab70882) at 1/100 dilution + mouse spleen tissue lysate at 12.5 µgSecondaryHRP-anti-rabbit

Anti-JNK3 antibody - ab87404 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-JNK3 antibody (ab87404) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 3 : Mouse Hippocampus Tissue Lysate Lane 4 : Cerebellum Mouse Tissue LysateLane 5 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate Lane 6 : Rat Hippocampus Tissue LysateLane 7 : Cerebellum Rat Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Anti-JNK3 antibody - ab137316 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-JNK3 antibody (ab137316) at 1/500 dilution + HUVEC cell lysate at 30 µg

Anti-JNK3 antibody [2G12H3] - ab52267 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G12H3
All lanes : Anti-JNK3 antibody [2G12H3] (ab52267) at 1/500 dilutionLane 1 : recombinant MAPK10Lane 2 : NIH3T3 cells

Anti-JNK3 antibody [EPR5493(2)] - ab126591 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5493(2)
Overlay histogram showing SHSY-5Y cells stained with ab126591 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126591, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MAPK10 / JNK3 Antibody (aa1-30, Azide-free, HRP) - LS-C262597 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPK10 / JNK3 Antibody (aa1-30, PE) - LS-C262598 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPK10 / JNK3 Antibody (N-Terminus, APC) - LS-C279222 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPK10 / JNK3 Antibody (aa361-410) - LS-C118941 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HeLa/293/Jurkat cells, using MAPK10 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-MAPK10 / JNK3 Antibody (N-Terminus, FITC) - LS-C279224 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPK10 / JNK3 Antibody (N-Terminus, Azide-free, HRP) - LS-C279225 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPK10 / JNK3 Antibody (N-Terminus, Biotin) - LS-C279223 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPK10 / JNK3 Antibody (N-Terminus, AP) - LS-C279221 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPK10 / JNK3 Antibody (N-Terminus, PE) - LS-C279226 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAPK10 / JNK3 Antibody (clone 8A5D11) - LS-C47194 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8A5D11

Anti-MAPK10 / JNK3 Antibody (aa1-30) - LS-C100712 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-MAPK10 / JNK3 Antibody - LS-C192875 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of various cell lines, using JNK3/MAPK10 antibody.

Anti-MAPK10 / JNK3 Antibody (clone 10E4A4) - LS-C108837 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10E4A4
Immunofluorescence staining of methanol-fixed A431 (left) and HeLa (right) cells showing cytoplasmic and membrane localization.

Anti-MAPK10 / JNK3 Antibody (aa88-332, Biotin) - LS-C299060 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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