Anti-KAP1 (phospho S824) antibody

• Supplier: Abcam
• Catalog: ab70369
• Prices: $394.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Human, Chimpanzee, Monkey, Ape, Orangutan
• Antigen: A synthetic phosphorylated peptide, corresponding to a sequence of human KAP1 surrounding Serine 824
• Description: Rabbit Polyclonal
• Gene: TRIM28
• Conjugate: Unconjugated

Product images

All lanes : Anti-KAP1 (phospho S824) antibody (ab70369) at 0.1 µg/mlLane 1 : Whole cell lysate from asynchronous HeLa cells, treated with neocarzinostatin (NCS; 200 ng/ml, 30 minutes) at 50 µgLane 2 : Whole cell lysate from asynchronous HeLa cells at 50 µgLane 3 : Whole cell lysate from asynchronous HeLa cells, treated with neocarzinostatin (NCS; 200 ng/ml, 30 minutes) at 200 µgLane 4 : Whole cell lysate from asynchronous HeLa cells at 200 µgdeveloped using the ECL technique

ab70369 staining KAP1 (phospho S824) (red) in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were treated with 1 µM Etoposide for 3 hours prior to fixing. Cells were fixed with formaldehyde, permeabilized in 0.5% NP40 and blocked with 3% BSA for 2 hours at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 3% BSA) for 12 hours at 4°C. An Alexa Fluor®594-conjugated Mouse anti-rabbit IgG monoclonal (1/500) was used as the secondary antibody.See Abreview

Product References

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