TRIM28

Gene Symbol TRIM28
Entrez Gene 10155
Alt Symbol KAP1, PPP1R157, RNF96, TF1B, TIF1B
Species Human
Gene Type protein-coding
Description tripartite motif containing 28
Other Description E3 SUMO-protein ligase TRIM28|KAP-1|KRAB [Kruppel-associated box domain]-associated protein 1|KRAB-associated protein 1|KRAB-interacting protein 1|KRIP-1|RING finger protein 96|TIF1-beta|nuclear corepressor KAP-1|protein phosphatase 1, regulatory subunit 157|transcription intermediary factor 1-beta|transcriptional intermediary factor 1-beta|tripartite motif-containing 28|tripartite motif-containing protein 28
Swissprots Q13263 Q96IM1 Q7Z632 Q93040 O00677
Accessions EAW72605 EAW72606 EAW72607 Q13263 AK131063 AK225760 AK313478 BAG36262 AL360114 AL360188 AL360189 BC004978 AAH04978 BC007390 AAH07390 BC052986 AAH52986 U31657 AAA74954 U78773 AAB37341 U95040 AAB51517 X97548 CAA66150 NM_005762 NP_005753
Function Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity towar
Subcellular Location Nucleus {ECO:0000269|PubMed:10330177, ECO:0000269|PubMed:17178852, ECO:0000269|PubMed:20858735, ECO:0000269|PubMed:23665872, ECO:0000269|PubMed:9016654}. Note=Associated with centromeric heterochromatin during cell differentiation through CBX1. {ECO:0000250}.
Tissue Specificity Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. {ECO:0000269|PubMed:9016654}.

Phospho-TIF1β (Ser824) Antibody - 4127 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or treated with ionizing radiation, using Phospho-TIF1β (Ser824) Antibody (upper) and TIF1β Antibody #4123 (lower).

TIF1β Antibody - 4123 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, A431 and PC3 cells using TIF1β antibody.

TIF1β (4E1) Mouse mAb - 5868 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 4E1
Western blot analysis of extracts from various cell lines using TIF1β (4E1) Mouse mAb.

TIF1β (C42G12) Rabbit mAb - 4124 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C42G12
Western blot analysis of extracts of HeLa and OVCAR3 cells using TIF1β (C42G12) Rabbit mAb.

TIF1β (H-300) - sc-33186 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TIF1β (H-300): sc-33186. Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.

TIF1β (C-16) - sc-19168 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TIF1β (Y-16) - sc-19169 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TIF1β (Y-16): sc-19169. Western blot analysis of TIF1β expression in non-transfected 293T: sc-117752 (A), human TIF1β transfected 293T: sc-116358 (B) and CCRF-CEM (C) whole cell lysates.

TIF1β (23) - sc-136102 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
TIF1β (23): sc-136102. Western blot analysis of TIF1β expression in rat pituitary tissue extract.

Anti- KAP1 antibody - ab43760 from Abcam

Prices $359.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti- KAP1 antibody (ab43760) at 1/1000 dilutionLane 1 : Total protein extract from E coli with ~50ng to 100ng of GST fusion protein of an irrelevant antigen at 20 µgLane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein)SecondaryRabbit anti-mouse IgG + IgM, (H+L) conjugated to Horseradish peroxidase at 1/5000 dilution

Anti-KAP1 (phospho S824) antibody - ab70369 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-KAP1 (phospho S824) antibody (ab70369) at 0.1 µg/mlLane 1 : Whole cell lysate from asynchronous HeLa cells, treated with neocarzinostatin (NCS; 200 ng/ml, 30 minutes) at 50 µgLane 2 : Whole cell lysate from asynchronous HeLa cells at 50 µgLane 3 : Whole cell lysate from asynchronous HeLa cells, treated with neocarzinostatin (NCS; 200 ng/ml, 30 minutes) at 200 µgLane 4 : Whole cell lysate from asynchronous HeLa cells at 200 µgdeveloped using the ECL technique

Anti-KAP1 (phospho S824) antibody - ab84077 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84077 at 1/250 dilution staining KIP1 in Neocarzinostatin treated asynchronous HeLa cells  by Immunocytochemistry / Immunofluorescence. A FITC conjugated anti rabbit used at a dilution of 1/100 as secondary.

Anti-KAP1 (phospho S824) antibody [ EPR5248] - ab133440 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5248
All lanes : Anti-KAP1 (phospho S824) antibody [ EPR5248] (ab133440) at 1/50000 dilutionLane 1 : Lysate of HeLa cells, untreated Lane 2 : Lysate of HeLa cells treated with gamma radiationLysates/proteins at 10 µg per lane.SecondaryHRP conjugated goat anti-rabbit at 1/2000 dilution

Anti-KAP1 antibody - ab10484 from Abcam

Prices $400.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and ovarian carcinoma (right) tissues labelling KAP1 with ab10484 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.

Anti-KAP1 antibody - ab3472 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of KAP1 (green) showing staining in the nucleus of L6 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3472 in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor® 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

Anti-KAP1 antibody - ab56587 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
KAP1 antibody (ab56587) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.

Anti-KAP1 antibody - ab84076 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84076 at a 1/100 dilution, staining KAP1 in formaldehyde fixed, paraffin embedded human skin tumor sections by immunohistochemistry. Detection: by DAB staining.

Anti-KAP1 antibody - ab88320 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining of formalin-fixed paraffin-embedded fetal small intestine showing nuclear staining with ab88320 dilution at 1/100 (A) and with blocking peptide (B)

Anti-KAP1 antibody [20C1] - ChIP Grade - ab22553 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 20C1
Anti-KAP1 antibody [20C1] - ChIP Grade (ab22553) at 1/500 dilution + HeLa whole cell lysateSecondaryGoat anti-mouse-HRP at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-KAP1 antibody [EPR5216] - ab109287 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5216
All lanes : Anti-KAP1 antibody [EPR5216] (ab109287) at 1/10000 dilutionLane 1 : HeLa cell lysatesLane 2 : A431 cell lysatesLane 3 : PC-3 cell lysatesLane 4 : MCF7 cell lysatesLysates/proteins at 10 µg per lane.

Anti-KAP1 antibody [EPR5217] - ab109289 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5217
All lanes : Anti-KAP1 antibody [EPR5217] (ab109289) at 1/1000 dilutionLane 1 : A431 cell lysateLane 2 : HeLa cell lysateLane 3 : PC-3 cell lysateLane 4 : F9 cell lysateLysates/proteins at 10 µg per lane.

Anti-KAP1 antibody [EPR5249] - ab109545 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5249
All lanes : Anti-KAP1 antibody [EPR5249] (ab109545) at 1/50000 dilutionLane 1 : A431 cell lysateLane 2 : A431 cell lysate treated with Lambda PhosphataseLane 3 : MCF7 cell lysateLane 4 : MCF7 cell lysate treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.

Anti-KAP1 antibody [TRI5I093] - ab50885 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone TRI5I093
Anti-KAP1 antibody [TRI5I093] (ab50885) at 1/50 dilution + HT1080 whole cell lysate at 25 µgSecondaryAnti mouse IgG antibody at 1/2500 dilution

Anti-KAP1 antibody - ChIP Grade - ab10483 from Abcam

Prices $404.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling KAP1 with ab10483 at 1/1000 (1μg/ml). Detection: DAB.

Anti-KAP1 antibody - ChIP Grade - ab3831 from Abcam

Prices $391.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-TRIM28 / KAP1 Antibody (C-Terminus) IHC-plus™ - LS-B10061 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TRIM28 / KAP-1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10061 concentration 10 ug/ml.
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