Anti-KAP1 antibody [20C1] - ChIP Grade
| Name | Anti-KAP1 antibody [20C1] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab22553 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | 20C1 |
| Applications | ChIP IHC-P ICC/IF ICC/IF WB EMSA IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 60-383 of Human KAP1 |
| Description | Mouse Monoclonal |
| Gene | TRIM28 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-KAP1 antibody [20C1] - ChIP Grade (ab22553) at 1/500 dilution + HeLa whole cell lysateSecondaryGoat anti-mouse-HRP at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/11342_ab22553-214846-22553-WB.jpg)
Anti-KAP1 antibody [20C1] - ChIP Grade (ab22553) at 1/500 dilution + HeLa whole cell lysateSecondaryGoat anti-mouse-HRP at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunofluorescence analysis of A549 cells, staining KAP1 (green) with ab22553. Cells were fixed in 4% paraformaldehyde followed by permeabilization in 0.1% Triton X-100/PBS. Cells were incubated in primary antibody and a FITC-conjugated goat anti-mouse secondary antibody was used to detect staining. Nuclei were stained with DAPI (blue).
IHC image of KAP1 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22553, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells. - hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells.
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Direct activation of ATM by resveratrol under oxidizing conditions. - Direct activation of ATM by resveratrol under oxidizing conditions.
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Inhibition of KAP1 enhances hypoxia-induced Kaposi's sarcoma-associated - Inhibition of KAP1 enhances hypoxia-induced Kaposi's sarcoma-associated
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Kaposi's sarcoma-associated herpesvirus-encoded LANA interacts with host KAP1 to - Kaposi's sarcoma-associated herpesvirus-encoded LANA interacts with host KAP1 to
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Genetic and epigenetic mutations affect the DNA binding capability of human ZFP57 - Genetic and epigenetic mutations affect the DNA binding capability of human ZFP57
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Distinct roles of KAP1, HP1 and G9a/GLP in silencing of the two-cell-specific - Distinct roles of KAP1, HP1 and G9a/GLP in silencing of the two-cell-specific
Maksakova IA, Thompson PJ, Goyal P, Jones SJ, Singh PB, Karimi MM, Lorincz MC. Epigenetics Chromatin. 2013 Jun 4;6(1):15.
A unique SUMO-2-interacting motif within LANA is essential for KSHV latency. - A unique SUMO-2-interacting motif within LANA is essential for KSHV latency.
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Tripartite motif containing 28 (Trim28) can regulate cell proliferation by - Tripartite motif containing 28 (Trim28) can regulate cell proliferation by
Chen L, Chen DT, Kurtyka C, Rawal B, Fulp WJ, Haura EB, Cress WD. J Biol Chem. 2012 Nov 23;287(48):40106-18.
MAGE I transcription factors regulate KAP1 and KRAB domain zinc finger - MAGE I transcription factors regulate KAP1 and KRAB domain zinc finger
Xiao TZ, Bhatia N, Urrutia R, Lomberk GA, Simpson A, Longley BJ. PLoS One. 2011;6(8):e23747.
MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases. - MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
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