Anti-KAP1 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab10483
• Prices: $404.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP IHC-P ICC/IF ICC/IF ICC/IF ChIP ChIP ChIP
• Species Reactivities: Mouse, Human
• Antigen: Immunogen was a synthetic peptide, which represented a portion of human tripartite motif-containing 28 (KAP1) encoded within exon 1 (LocusLink ID 10155)
• Description: Rabbit Polyclonal
• Gene: TRIM28
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling KAP1 with ab10483 at 1/1000 (1μg/ml). Detection: DAB.

Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling KAP1 with ab10483 at 1/1000 (1μg/ml). A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.

The ab10483 antibody was used to immunoprecipitate KAP1 from HEK293 nuclear extracts. Three different amounts of ab10483 or control IgG were tested (1, 2 or 4 ug). The eluates from these experiments as well as the supernatants from the KAP1 IPs were analyzed by Western blotting using the ab10483 antibody. As shown by Western blotting, the presence of a ~110 kDa band demonstrated that KAP1 was specifically precipitated from these extracts. The 50 and 25 kDa bands correspond to the IgG of the IP antibodies.See Abreview

IHC image of KAP1 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10483, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression - SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression

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