ab10308 Immunoprecipitate KAT13A / SRC1 in human Huh-7 whole cell lysate. 1,000,000 cells were lysed and incubated with primary antibody at 4µg/mg lysate and Protein A matrix for 20 hours at 4°C. For western blotting an undiluted Abcam`s ab2859, Rabbit polyclonal to KAT13A / SRC1 was used.See Abreview
ab10308 used in Immunoprecipitation.Total protein input is 1 x 106 cells.Whole cell lysate was prepared from VERO cells expressing recombinant SRC1 protein.Protein A matrix was used for the Immunoprecipitation step.ab10308 used a 4µg/mg lysate for 16 hours at 4°C.ab2859 was then used for Western Blot at 1µg/mg lysate.See Abreview