Anti-KAT13D / CLOCK antibody [EPR6227]

• Supplier: Abcam
• Catalog: ab134165
• Prices: $395.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6227
• Applications: WB IP IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in Human KAT13D/ CLOCK (O15516)
• Description: Rabbit Monoclonal
• Gene: CLOCK
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab134165 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134165, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-KAT13D / CLOCK antibody [EPR6227] (ab134165) at 1/10000 dilutionLane 1 : 293T cell lysatesLane 2 : PC-3 cell lysatesLane 3 : HeLa cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin embedded Human colon tissue labelled with ab134165 at 1/100 dilution.

Immunofluorescence analysis of HeLa cells labelled with ab134165 at 1/250 dilution.