CLOCK

Gene Symbol CLOCK
Entrez Gene 9575
Alt Symbol KAT13D, bHLHe8
Species Human
Gene Type protein-coding
Description clock circadian regulator
Other Description circadian locomoter output cycles kaput protein|circadian locomoter output cycles protein kaput|class E basic helix-loop-helix protein 8|clock homolog
Swissprots A0AV01 Q9UIT8 O14516 A2I2N9 O15516
Accessions AAF70307 ABM64208 BAB88852 BAB92020 EAX05468 O15516 AB002332 BAA20792 AB005535 BAA21774 AF011568 AAB83969 AK096319 AK124437 AK223549 BAD97269 AK291708 BAF84397 BC040570 BC041878 AAH41878 BC126157 AAI26158 BC126159 AAI26160 BX537616 BX538337 DA658950 EU446690 ABZ92219 HQ258670 ADR83421 XM_005265787 XP_005265844 XM_006714054 XP_006714117 XM_011534409 XP_011532711 XM_011534410 XP_011532712 XM_011534411 XP_011532713 NM_001267843 NP_001254772 NM_004898 NP_004889
Function Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time- keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and
Subcellular Location Nucleus {ECO:0000269|PubMed:14645221, ECO:0000269|PubMed:23160374}. Cytoplasm {ECO:0000250|UniProtKB:O08785}. Note=Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL/BMAL1-dependent. Phosphorylated form located in the nucleus while the nonphosphorylated form found only in the cytoplasm. Sequestered to the cytoplasm in the presence of ID2 (By similarity). Localizes to sites of DNA damage in a H2AX- independent manner. {ECO:0000250|UniProtKB:O08785, ECO:0000269|PubMed:21659603}.
Tissue Specificity Hair follicles (at protein level). Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN). {ECO:0000269|PubMed:10198158, ECO:0000269|PubMed:24005054}.
Top Pathways Circadian rhythm, Herpes simplex infection, Dopaminergic synapse

CLOCK (D45B10) Rabbit mAb - 5157 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D45B10
Western blot analysis of extracts from various cell lines using CLOCK (D45B10) Rabbit mAb.

Clock (C-8) - sc-271603 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Clock (C-8): sc-271603. Western blot analysis of Clock expression in F9 whole cell lysate.

Clock (M-20) - sc-6928 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Clock (H-276) - sc-25361 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Clock (H-276): sc-25361. Western blot analysis of Clock expression in Sol8 whole cell lysate.

Clock (S-19) - sc-6927 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Clock (S-19): sc-6927. Western blot analysis of Clock expression in rat hypothalamus (A) and rat brain (B) tissue extracts.

Anti-KAT13D / CLOCK antibody - ab65033 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab65033, at a 1/50 dilution, staining KAT13D / CLOCK in paraffin embedded human skeletal muscle tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

Anti-KAT13D / CLOCK antibody - ab93804 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-KAT13D / CLOCK antibody (ab93804) at 0.1 µg/mlLane 1 : HeLa at 50 µgLane 2 : HeLa at 15 µgLane 3 : HeLa at 5 µgLane 4 : 293T at 50 µgdeveloped using the ECL technique

Anti-KAT13D / CLOCK antibody - ab93805 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab93805 at 1 µg/ml detecting KAT13D/CLOCK in HeLa whole cell lysate by WB following IP. Lane 1: ab93805 at 3µg/mg of lysate Lane 2: IP with an antibody which recognizes an downstream epitope of KAT13D/CLOCK Lane 3: control IgG. In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded. Detection: Chemiluminescence with an exposure time of 30 seconds

Anti-KAT13D / CLOCK antibody [648C1a] - ab51333 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 648C1a

Anti-KAT13D / CLOCK antibody [8F7] - ab98948 from Abcam

Host Mouse
Clonality Monoclonal
Clone 8F7
Anti-KAT13D / CLOCK antibody [8F7] (ab98948) at 1/500 dilution + KAT13D-CLOCK transfected HEK293 cell lysate

Anti-KAT13D / CLOCK antibody [EPR6227] - ab134165 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6227
Overlay histogram showing HeLa cells stained with ab134165 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134165, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-KAT13D / CLOCK antibody - ChIP Grade - ab3517 from Abcam

Prices $400.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/immunofluorescence analysis of U251 cells labeling KAT13D/CLOCK (green) with ab3517 at 1/100. Cells were fixed with formalin and permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blcoked with £% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. F-actin (red) was stained with phalloidin and nuclei (blue) were stained with Hoechst or DAPI. 60X magnification. Left - negative control.

Anti-CLOCK Antibody (N-Terminus) - LS-C46809 from LifeSpan Bioscience

Prices $295.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody - LS-C125373 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody - LS-C137019 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody (aa805-855, Biotin) - LS-C143177 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody (aa805-855, DY650) - LS-C143178 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody (aa805-855, DY488) - LS-C143179 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody (aa805-855, HRP) - LS-C143180 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody (aa805-855, DY550) - LS-C143181 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody - LS-C147021 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody (clone 648C1a) - LS-C184450 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 648C1a

Anti-CLOCK Antibody (aa450-500) - LS-C289348 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLOCK Antibody (N-Terminus) - LS-C109824 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CLOCK antibody LS-C109824 Western blot of 721_B cell lysate.

Anti-CLOCK Antibody (clone 8F7) - LS-C109148 from LifeSpan Bioscience

Prices $320.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8F7
Immunofluorescence of HeLa cells using CLOCK mouse monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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