Anti-KAT13D / CLOCK antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab3517
• Prices: $400.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P IHC-F WB EMSA ChIP ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Hamster, Human
• Antigen: Synthetic peptide corresponding to Mouse KAT13D/ CLOCK aa 11-30
• Description: Rabbit Polyclonal
• Gene: CLOCK
• Conjugate: Unconjugated

Product images

Immunocytochemistry/immunofluorescence analysis of U251 cells labeling KAT13D/CLOCK (green) with ab3517 at 1/100. Cells were fixed with formalin and permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blcoked with £% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. F-actin (red) was stained with phalloidin and nuclei (blue) were stained with Hoechst or DAPI. 60X magnification. Left - negative control.

ab3517 labelling KAT13D in the nucleus and cytoplasm of Human skeletal muscle tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Thissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ab3517 labelling KAT13D in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Thissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ab3517 labelling KAT13D in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Thissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ab3517 staining KAT13D/CLOCK in Mouse skeletal muscle tissue sections by IHC-P (Paraformaldehyde-fixed, paraffin-embedded tissue sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer pH6. Samples were incubated with primary antibody (1/400 in PBS) for 12 hours at 4°C. Undiluted ab64256 was used as the secondary antibody.See Abreview

All lanes : Anti-KAT13D / CLOCK antibody - ChIP Grade (ab3517) at 1/4000 dilutionLane 1 : HeLa cell lysateLane 2 : NIH-3T3 cell lysateLysates/proteins at 25 µg per lane.

All lanes : Anti-KAT13D / CLOCK antibody - ChIP Grade (ab3517) at 1 µg/mlLane 1 : Skeletal Muscle (Mouse) Tissue Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

Epigenetic suppression of mouse Per2 expression in the suprachiasmatic nucleus by - Epigenetic suppression of mouse Per2 expression in the suprachiasmatic nucleus by

Mori K, Iijima N, Higo S, Aikawa S, Matsuo I, Takumi K, Sakamoto A, Ozawa H. PLoS One. 2014 Jan 31;9(1):e87319.

Expressions of tight junction proteins Occludin and Claudin-1 are under the - Expressions of tight junction proteins Occludin and Claudin-1 are under the

Kyoko OO, Kono H, Ishimaru K, Miyake K, Kubota T, Ogawa H, Okumura K, Shibata S, Nakao A. PLoS One. 2014 May 20;9(5):e98016.

Linking the circadian rhythm gene Arntl2 to interleukin 21 expression in type 1 - Linking the circadian rhythm gene Arntl2 to interleukin 21 expression in type 1

Lebailly B, He C, Rogner UC. Diabetes. 2014 Jun;63(6):2148-57.

Circadian clock function is disrupted by environmental tobacco/cigarette smoke, - Circadian clock function is disrupted by environmental tobacco/cigarette smoke,

Hwang JW, Sundar IK, Yao H, Sellix MT, Rahman I. FASEB J. 2014 Jan;28(1):176-94.

A non-canonical E-box within the MyoD core enhancer is necessary for circadian - A non-canonical E-box within the MyoD core enhancer is necessary for circadian

Zhang X, Patel SP, McCarthy JJ, Rabchevsky AG, Goldhamer DJ, Esser KA. Nucleic Acids Res. 2012 Apr;40(8):3419-30.

Non-invasive exploration of neonatal gastric epithelium by using exfoliated - Non-invasive exploration of neonatal gastric epithelium by using exfoliated

Kaeffer B, Legrand A, Moyon T, Frondas-Chauty A, Billard H, Guzman-Quevedo O, Darmaun D, Roze JC. PLoS One. 2011;6(10):e25562.

CLOCK and BMAL1 regulate MyoD and are necessary for maintenance of skeletal - CLOCK and BMAL1 regulate MyoD and are necessary for maintenance of skeletal

Andrews JL, Zhang X, McCarthy JJ, McDearmon EL, Hornberger TA, Russell B, Campbell KS, Arbogast S, Reid MB, Walker JR, Hogenesch JB, Takahashi JS, Esser KA. Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):19090-5. doi:

Myocyte remodeling in response to hypertrophic stimuli requires nucleocytoplasmic - Myocyte remodeling in response to hypertrophic stimuli requires nucleocytoplasmic

Boateng SY, Senyo SE, Qi L, Goldspink PH, Russell B. J Mol Cell Cardiol. 2009 Oct;47(4):426-35.

Circadian timing in the lung; a specific role for bronchiolar epithelial cells. - Circadian timing in the lung; a specific role for bronchiolar epithelial cells.

Gibbs JE, Beesley S, Plumb J, Singh D, Farrow S, Ray DW, Loudon AS. Endocrinology. 2009 Jan;150(1):268-76.

The circadian protein Clock localizes to the sarcomeric Z-disk and is a sensor of - The circadian protein Clock localizes to the sarcomeric Z-disk and is a sensor of

Qi L, Boateng SY. Biochem Biophys Res Commun. 2006 Dec 29;351(4):1054-9. Epub 2006 Nov 7.