Anti-KAT5 / Tip60 antibody
| Name | Anti-KAT5 / Tip60 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23886 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Drosophila, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Tip60 |
| Blocking Peptide | Human KAT5 / Tip60 peptide |
| Description | Rabbit Polyclonal |
| Gene | KAT5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab23886 staining KAT5 / Tip60 in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 2 hours. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
HeLa cells were plated on poly-D lysine coated cover slips, fixed with 4% w/v paraformaldehyde (10 min), permeabilised in 0.1% Triton X-100 in PBS (10 min), incubated for 1 hour at 37°C with ab23886 and stained with a FITC-conjugated secondary antibody and DAPI. Left panel: FITC signal. Right panel: FITC+DAPI.ab23886 gives a specific nuclear staining for Tip60. Nucleoli are stained more intensely, as also demonstrated by co-staining with known nucleolar makers (not shown). The signal is blocked by pre-incubation of ab23886 with the cognate immunogenic peptide.
ICC/IF image of ab23886 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23886, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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