Anti-KAT8 / MYST1 / MOF antibody [EPR15803]

• Supplier: Abcam
• Catalog: ab200660
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15803
• Applications: IHC-P WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KAT8/ MYST1/ MOF aa 400 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: KAT8
• Conjugate: Unconjugated

Product images

All lanes : Anti-KAT8 / MYST1 / MOF antibody [EPR15803] (ab200660) at 1/5000 dilutionLane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-KAT8 / MYST1 / MOF antibody [EPR15803] (ab200660) at 1/1000 dilution + Human fetal kidney lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-KAT8 / MYST1 / MOF antibody [EPR15803] (ab200660) at 1/1000 dilutionLane 1 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling KAT8 / MYST1 / MOF with ab200660 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Nuclear staining on Human cervix carcinoma tissue is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling KAT8 / MYST1 / MOF with ab200660 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.Nuclear staining on rat kidney tissue is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

KAT8 / MYST1 / MOF was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200660 at 1/170 dilution.Western blot was performed from the immunoprecipitate using ab200660 at 1/1000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell lysate 10 µg (Input).Lane 2: ab200660 IP in HeLa whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200660 in HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.