Lane 1 : Anti-KCNK18/TRESK antibody (ab106411) at 1 µg/mlLane 2 : Anti-KCNK18/TRESK antibody (ab106411) at 2 µg/mlLane 1 : Rat brain tissue lysate Lane 2 : Rat brain tissue lysate Lysates/proteins at 15 µg per lane.
ab106411, at 5 µg/ml, staining KCNK18/TRESK in paraffin embedded Rat brain tissue.
ICC/IF image of ab106411 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106411, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of TRESK in Rat Brain cells using ab106411 at 20 ug/ml.