Anti-KCNN4 antibody - C-terminal
| Name | Anti-KCNN4 antibody - C-terminal |
|---|---|
| Supplier | Abcam |
| Catalog | ab83740 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide from the C terminal region of human KCNN4 conjugated to an immunogenic carrier protein |
| Description | Rabbit Polyclonal |
| Gene | KCNN4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-KCNN4 antibody - C-terminal (ab83740) at 1/500 dilution + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab83740 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83740, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Intermediate-conductance Ca2+-activated K+ channel, KCa3.1, as a novel - Intermediate-conductance Ca2+-activated K+ channel, KCa3.1, as a novel
Ohya S, Niwa S, Kojima Y, Sasaki S, Sakuragi M, Kohri K, Imaizumi Y. J Pharmacol Exp Ther. 2011 Aug;338(2):528-36.
Ion channels in the regulation of platelet migration. - Ion channels in the regulation of platelet migration.
Schmidt EM, Munzer P, Borst O, Kraemer BF, Schmid E, Urban B, Lindemann S, Ruth P, Gawaz M, Lang F. Biochem Biophys Res Commun. 2011 Nov 11;415(1):54-60. doi:
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Liang Z, Chen L, McClafferty H, Lukowski R, MacGregor D, King JT, Rizzi S, Sausbier M, McCobb DP, Knaus HG, Ruth P, Shipston MJ. J Physiol. 2011 Dec 15;589(Pt 24):5965-86.