Anti-KCNQ2 antibody
| Name | Anti-KCNQ2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab22897 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human KCNQ2 |
| Description | Rabbit Polyclonal |
| Gene | KCNQ2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-KCNQ2 antibody (ab22897) at 1 µg/mlLane 1 : Rat whole brain lysateLane 2 : Mouse whole brain lysateLysates/proteins at 40 µg per lane.SecondaryAnti-rabbit HRP at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunfluorescent detection of KCNQ2 (green colour) in differentiated murine neural precursors using Rabbit polyclonal to KCNQ2 (ab22897). KCNQ2 labeling is seen in the tips of neurite extensions (such as the cell in the top left corner), along axons, dendrites and possibily spines. ab22897 (3.5µg/ml) was incubated overnight at 4°C. Secondary antibody used is goat anti-rabbit Alexa fluor 488 (1h at RT ). The red colour is MAP2 detection (Secondary used is goat anti-mouse Alexa fluor 568; 1h at RT). NB: The white centres are caused by high intensity to-pro-3 blue nuclear counterstain cross-colouring with the red and background green.
All lanes : Anti-KCNQ2 antibody (ab22897) at 1 µg/mlLane 1 : Brain (Rat) Tissue LysateLane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Human) Tissue Lysate - adult normal tissueLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
The kick-in system: a novel rapid knock-in strategy. - The kick-in system: a novel rapid knock-in strategy.
Tomonoh Y, Deshimaru M, Araki K, Miyazaki Y, Arasaki T, Tanaka Y, Kitamura H, Mori F, Wakabayashi K, Yamashita S, Saito R, Itoh M, Uchida T, Yamada J, Migita K, Ueno S, Kitaura H, Kakita A, Lossin C, Takano Y, Hirose S. PLoS One. 2014 Feb 19;9(2):e88549.
Coexpression and activation of TRPV1 suppress the activity of the KCNQ2/3 - Coexpression and activation of TRPV1 suppress the activity of the KCNQ2/3
Zhang XF, Han P, Neelands TR, McGaraughty S, Honore P, Surowy CS, Zhang D. J Gen Physiol. 2011 Sep;138(3):341-52.
Protein Phosphatase 2a and glycogen synthase kinase 3 signaling modulate prepulse - Protein Phosphatase 2a and glycogen synthase kinase 3 signaling modulate prepulse
Kapfhamer D, Berger KH, Hopf FW, Seif T, Kharazia V, Bonci A, Heberlein U. J Neurosci. 2010 Jun 30;30(26):8830-40.