![Overlay histogram showing SH-SH5Y cells stained with ab84812 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84812, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/11940_ab84812-4-ab84812FC.jpg)
Overlay histogram showing SH-SH5Y cells stained with ab84812 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84812, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-KCNQ2 antibody [S26A-23] (ab84812) at 10 µg/mlLane 1 : CHO cell lysateLane 2 : HEK cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/11941_ab84812-199518-ab84812wb.jpg)
All lanes : Anti-KCNQ2 antibody [S26A-23] (ab84812) at 10 µg/mlLane 1 : CHO cell lysateLane 2 : HEK cell lysate
ab84812 staining KCNQ2 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
ab84812 staining KCNQ2 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).