Anti-KDM1 / LSD1 antibody - ChIP Grade
| Name | Anti-KDM1 / LSD1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab17721 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP IHC-P IP ICC/IF ICC/IF WB IHC-F |
| Species Reactivities | Mouse, Human, Pig |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human LSD1 |
| Blocking Peptide | Human KDM1 / LSD1 peptide |
| Description | Rabbit Polyclonal |
| Gene | KDM1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab17721 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab17721, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab17721 at a 1/100 dilution in cross-linking ChIP (using formaldehyde) with mouse liver tissue lysate. The protocol from Nguyen, T. T, et al. (2005) Mol. Cell. Biol. 25: 2147-2157 was followed. Semiquantitative PCR was used as the detection step. Lane 1: InputLane 2: ab17721Lane 3: IgGLane 4: No abThis image is courtesy of an Abreview by Michelle Barton.See Abreview
All lanes : Anti-KDM1 / LSD1 antibody - ChIP Grade (ab17721) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilutionPerformed under reducing conditions.
Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab17721 (1/200) for LSD1 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.
Product References
An H2A histone isotype regulates estrogen receptor target genes by mediating - An H2A histone isotype regulates estrogen receptor target genes by mediating
Su CH, Tzeng TY, Cheng C, Hsu MT. Nucleic Acids Res. 2014 Mar;42(5):3073-88.
LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone - LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone
Yin F, Lan R, Zhang X, Zhu L, Chen F, Xu Z, Liu Y, Ye T, Sun H, Lu F, Zhang H. Mol Cell Biol. 2014 Jan;34(2):158-79.
A comparison of the rest complex binding patterns in embryonic stem cells and - A comparison of the rest complex binding patterns in embryonic stem cells and
Seki M, Masaki H, Arauchi T, Nakauchi H, Sugano S, Suzuki Y. PLoS One. 2014 Apr 21;9(4):e95374.
Antagonistic actions of Rcor proteins regulate LSD1 activity and cellular - Antagonistic actions of Rcor proteins regulate LSD1 activity and cellular
Upadhyay G, Chowdhury AH, Vaidyanathan B, Kim D, Saleque S. Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8071-6. doi:
Identification of myelin transcription factor 1 (MyT1) as a subunit of the neural - Identification of myelin transcription factor 1 (MyT1) as a subunit of the neural
Yokoyama A, Igarashi K, Sato T, Takagi K, Otsuka I M, Shishido Y, Baba T, Ito R, Kanno J, Ohkawa Y, Morohashi K, Sugawara A. J Biol Chem. 2014 Jun 27;289(26):18152-62.
CtBP and associated LSD1 are required for transcriptional activation by NeuroD1 - CtBP and associated LSD1 are required for transcriptional activation by NeuroD1
Ray SK, Li HJ, Metzger E, Schule R, Leiter AB. Mol Cell Biol. 2014 Jun;34(12):2308-17.
Transcriptional regulation of the albumin gene depends on the removal of histone - Transcriptional regulation of the albumin gene depends on the removal of histone
Liu D, Zempleni J. J Nutr. 2014 Jul;144(7):997-1001.
High nuclear expression levels of histone-modifying enzymes LSD1, HDAC2 and SIRT1 - High nuclear expression levels of histone-modifying enzymes LSD1, HDAC2 and SIRT1
Derr RS, van Hoesel AQ, Benard A, Goossens-Beumer IJ, Sajet A, Dekker-Ensink NG, de Kruijf EM, Bastiaannet E, Smit VT, van de Velde CJ, Kuppen PJ. BMC Cancer. 2014 Aug 20;14:604.
Low activity of LSD1 elicits a pro-inflammatory gene expression profile in - Low activity of LSD1 elicits a pro-inflammatory gene expression profile in
Liu D, Zempleni J. Genes Nutr. 2014 Sep;9(5):422.
LSD1 controls metastasis of androgen-independent prostate cancer cells through - LSD1 controls metastasis of androgen-independent prostate cancer cells through
Ketscher A, Jilg CA, Willmann D, Hummel B, Imhof A, Russeler V, Holz S, Metzger E, Muller JM, Schule R. Oncogenesis. 2014 Oct 6;3:e120.