KDM1A

Gene Symbol KDM1A
Entrez Gene 23028
Alt Symbol AOF2, BHC110, KDM1, LSD1
Species Human
Gene Type protein-coding
Description lysine (K)-specific demethylase 1A
Other Description BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|amine oxidase (flavin containing) domain 2|flavin-containing amine oxidase domain-containing protein 2|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1|lysine-specific histone demethylase 1A
Swissprots Q5TH94 Q5TH95 O60341 Q8IXK4 A8MWP9 Q86VT7 Q96AW4 Q8TAZ3 Q8NDP6
Accessions CBY79414 CBY88366 EAW95031 EAW95032 EAW95033 O60341 AA664447 AB011173 BAA25527 AK128669 AK290328 BAF83017 AL833812 CAD38675 BC016639 AAH16639 BC025362 AAH25362 BC040194 AAH40194 BC048134 AAH48134 BG717076 BQ006926 XM_005245786 XP_005245843 XM_006710472 XP_006710535 XM_006710473 XP_006710536 XM_006710474 XP_006710537 NM_001009999 NP_001009999 NM_015013 NP_055828
Function Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag tha
Subcellular Location Nucleus {ECO:0000269|PubMed:11102443, ECO:0000269|PubMed:16079795}.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:16079795}.

LSD1 (C69G12) Rabbit mAb - 2184 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C69G12
Western blot analysis of cell lysates from various cell types using LSD1 (C69G12) Rabbit mAb.

LSD1 Antibody - 2139 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of cell lysates from various cell lines, using LSD1 Antibody.

LSD1 (1E5-H2) Mouse mAb - 4218 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1E5-H2
Western blot analysis of extracts from various cell types using LSD1 (1E-5H2) Mouse mAb.

LSD1 (1B2E5) Mouse mAb - 4064 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1B2E5
Western blot analysis of cell lysates from HeLa, NIH/3T3 and COS cells, using LSD1 (1B2E5) Mouse mAb.

LSD1 (A-2) - sc-398794 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LSD1 (A-2): sc-398794. Western blot analysis of LSD1 expression in SK-BR-3 (A), K-562 (B) and SW480 (C) nuclear extracts and Jurkat whole cell lysate (D).

LSD1 (B-9) - sc-271720 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LSD1 (B-9): sc-271720. Western blot analysis of LSD1 expression in Jurkat nuclear extract (A) and HeLa whole cell lysate (B).

LSD1 (H-220) - sc-67272 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LSD1 (H-220): sc-67272. Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing nuclear staining of ovarian stroma cells.

LSD1 (1B2E5) - sc-53875 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LSD1 (1B2E5): sc-53875. Western blot analysis of LSD1 expression in HeLa (A), PC-3 (B), Jurkat (C), SK-BR-3 (D) and SW480 (E) nuclear extracts.

LSD1 (P-20) - sc-49294 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LSD1 (P-20): sc-49294. Western blot analysis of LSD1 expression in Jurkat (A), SKBR-3 (B) and K-562 (C) nuclear extracts.

LSD1 (C-19) - sc-49291 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

LSD1 (2D6) - sc-136174 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LSD1 (2D6): sc-136174. Western blot analysis of LSD1 expression in HeLa (A), PC-3 (B), Jurkat (C), SK-BR-3 (D), SW480 (E) and MCF7 (F) nuclear extracts.

Anti-KDM1 / LSD1 antibody - ab37165 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of KDM1/LSD1 staining in human prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37165, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-KDM1 / LSD1 antibody - ab62582 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-KDM1 / LSD1 antibody (ab62582) at 1 µg/mlLane 2 : Anti-KDM1 / LSD1 antibody (ab62582) at 2 µg/mlLane 1 : P815 cell lysateLane 2 : P815 cell lysateLysates/proteins at 15 µg per lane.

Anti-KDM1 / LSD1 antibody - ab51877 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-KDM1 / LSD1 antibody (ab51877) at 1/2000 dilution + HeLa cell nuclear extract at 20 µg

Anti-KDM1 / LSD1 antibody - ab135496 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-KDM1 / LSD1 antibody (ab135496) at 1/100 dilutionLane 1 : 293 cell lysate, non-transfectedLane 2 : 293 cell lysate, transiently transfected with the KDM1 / LSD1 geneLysates/proteins at 2 µg per lane.

Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] - ab31954 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B2E5 + 1B2F2
All lanes : Anti-KDM1 / LSD1 antibody [1B2E5 + 1B2F2] (ab31954)Lane 1 : Molecular weight markerLane 2 : LSD1 recombinant protein (NB: truncated form)Lane 3 : HeLa cell lysate

Anti-KDM1 / LSD1 antibody [1B2F2] - ab69535 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1B2F2
All lanes : Anti-KDM1 / LSD1 antibody [1B2F2] (ab69535) at 1 µg/mlLane 1 : Molecular weight markerLane 2 : HeLa cell extractLane 3 : NIH/3T3 cell extractdeveloped using the ECL technique

Anti-KDM1 / LSD1 antibody [EPR6825] - Nuclear Envelope Marker - ab129195 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6825
All lanes : Anti-KDM1 / LSD1 antibody [EPR6825] - Nuclear Envelope Marker (ab129195) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : PC3 cell lysateLane 5 : C6 cell lysateLane 6 : RAW264.7 cell lysateLane 7 : PC12 cell lysateLane 8 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-KDM1 / LSD1 antibody [EPR6825] - Nuclear Envelope Marker (Alexa Fluor® 488) - ab184811 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6825
ab184811 staining KDM1/ LSD1 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab184811 at a working dilution of 1 in 100 overnight at +4°C (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410).
Overlay histogram showing HeLa cells stained with ab195304 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195304, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-KDM1 / LSD1 antibody [EPR6825] - Nuclear Envelope Marker (Alexa Fluor® 647) - ab195305 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6825
ab195305 staining KDM1 / LSD1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195305 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-KDM1 / LSD1 antibody [EPR6825] - Nuclear Envelope Marker (HRP) - ab195897 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6825
All lanes : Anti-KDM1 / LSD1 antibody [EPR6825] - Nuclear Envelope Marker (HRP) (ab195897) at 1/5000 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-KDM1 / LSD1 antibody [EPR6826] - ab125017 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6826
All lanes : Anti-KDM1 / LSD1 antibody [EPR6826] (ab125017) at 1/1000 dilutionLane 1 : T47D lysateLane 2 : Jurkat lysateLane 3 : HeLa lysateLane 4 : 293T lysateLane 5 : JAR lysateLane 6 : SW480 lysateLane 7 : PC3 lysateLane 8 : F9 lysateLane 9 : C6 lysateLane 10 : PC12 lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-KDM1 / LSD1 antibody - ChIP Grade - ab17721 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab17721 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab17721, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

Anti-KDM1 / LSD1 antibody - ChIP Grade - ab90966 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Ab90966 was tested for immunostaining (upper panel) and IP-Western (lower panel). For immunostaining analysis, HeLa cells were transfected with a shLSD1 plasmid also encoding GFP for two days followed by immunostaining using ab90966. For IP-Western analysis, HeLa nuclear extracts (~200ug for each reaction) were immunoprecipitated with or without 0.5ug LSD antibody followed by Western analysis at a dilution of 1:50.
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