![All lanes : Anti-KDM3A / JHDM2A antibody [1E12] (ab107234) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/12183_KDM3A-JHDM2A-Primary-antibodies-ab107234-1.jpg)
All lanes : Anti-KDM3A / JHDM2A antibody [1E12] (ab107234) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysate
Immunohistochemical analysis of KDM3A / JHDM2A in paraffin embedded Human colonic cancer tissue, using ab107234 at a dilution of 1/200 with DAB staining.
Immunohistochemical analysis of KDM3A / JHDM2A in paraffin embedded Human lung cancer tissue, using ab107234 at a dilution of 1/200 with DAB staining.
![Overlay histogram showing HeLa cells stained with ab107234 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107234, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/12186_KDM3A-JHDM2A-Primary-antibodies-ab107234-4.jpg)
Overlay histogram showing HeLa cells stained with ab107234 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107234, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.