KDM3A

Gene Symbol KDM3A
Entrez Gene 55818
Alt Symbol JHDM2A, JHMD2A, JMJD1, JMJD1A, TSGA
Species Human
Gene Type protein-coding
Description lysine (K)-specific demethylase 3A
Other Description jmjC domain-containing histone demethylation protein 2A|jumonji C domain-containing histone demethylase 2A|jumonji domain containing 1|jumonji domain containing 1A|jumonji domain-containing protein 1A|lysine-specific demethylase 3A|testis-specific protein A
Swissprots Q68D47 D6W5M3 Q8IY08 Q6N050 Q9Y4C1 Q68UT9 Q53S72
Accessions AAY24210 EAW99453 EAW99454 EAW99455 EAW99456 Q9Y4C1 AB018285 BAA34462 AI911797 AK074314 AK125045 AK304276 BAG65137 AL832150 CAH18459 BC038297 BC156518 AAI56519 BC172547 AAI72547 BG723267 BX537674 BX640698 CAE45820 BX647271 BX647752 BX648841 BX649109 CR749581 CAH18373 DB087171 DB453517 XM_006712051 XP_006712114 XM_011532984 XP_011531286 XM_011532985 XP_011531287 XM_011532986 XP_011531288 NM_001146688 NP_001140160 NM_018433 NP_060903
Function Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. {ECO:0000269|PubMed:16603237}.
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Note=Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity). {ECO:0000250}.

JMJD1A (C-6) - sc-376608 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
JMJD1A (C-6): sc-376608. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

JMJD1A (H-1) - sc-398946 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
JMJD1A (H-1): sc-398946. Western blot analysis of JMJD1A expression in F9 (A), HeLa (B) and MCF7 (C) whole cell lysates.

JMJD1A (H-300) - sc-292213 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

JMJD1A (T-14) - sc-107656 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
JMJD1A (T-14): sc-107656. Western blot analysis of JMJD1A expression in Jurkat whole cell lysate.

Anti-JMJD1A antibody - ab106456 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-JMJD1A antibody (ab106456) at 1 µg/mlLane 2 : Anti-JMJD1A antibody (ab106456) at 2 µg/mlLane 1 : Mouse liver tissue lysateLane 2 : Mouse liver tissue lysateLysates/proteins at 15 µg per lane.

Anti-KDM3A / JHDM2A antibody - ab80598 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/mlLane 1 : whole HeLa cell lysate at 50 µgLane 2 : whole HeLa cell lysate at 15 µgLane 3 : whole HeLa cell lysate at 5 µg

Anti-KDM3A / JHDM2A antibody [14F8] - ChIP Grade - ab91252 from Abcam

Prices $387.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 14F8
IHC image of KDM3A / JHDM2A antibody staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91252, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-KDM3A / JHDM2A antibody [1E12] - ab107234 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E12
All lanes : Anti-KDM3A / JHDM2A antibody [1E12] (ab107234) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysate

Anti-KDM3A / JHDM2A antibody [2E1] - ab107235 from Abcam

Host Mouse
Clonality Monoclonal
Clone 2E1
Overlay histogram showing HeLa cells stained with ab107235 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107235, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-KDM3A / JHDM2A antibody - Aminoterminal end - ab75620 from Abcam

Prices $379.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab75620, at a dilution of 1/50, staining KDM3A / JHDM2A in formalin fixed and paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.

Anti-KDM3A / JHDM2A antibody - C-terminal - ab157005 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-KDM3A / JHDM2A antibody - C-terminal (ab157005) at 1/1000 dilutionLane 1 : Jurkat total cell extractLane 2 : T47D total cell extract

Anti-KDM3A / JHDM2A antibody - N-terminal - ab135053 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-KDM3A / JHDM2A antibody - N-terminal (ab135053) at 1/1000 dilution + HeLa total cell extracts

Anti-KDM3A / JMJD1A Antibody (clone 1E12) - LS-C169283 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E12
IHC of paraffin-embedded colonic cancer tissues (left) and lung cancer tissues (right) using KDM3A mouse monoclonal antibody with DAB staining.

Anti-KDM3A / JMJD1A Antibody (clone 2E1) - LS-C169284 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E1
Immunofluorescence of HeLa cells using KDM3A mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Anti-KDM3A / JMJD1A Antibody - LS-C332062 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of A549 cell using KDM3A antibody.

Anti-KDM3A / JMJD1A Antibody (aa250-400, DY650) - LS-C143506 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KDM3A / JMJD1A Antibody (aa250-400, DY488) - LS-C143507 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KDM3A / JMJD1A Antibody (aa250-400, Biotin) - LS-C143505 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KDM3A / JMJD1A Antibody (aa250-400, HRP) - LS-C143508 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KDM3A / JMJD1A Antibody (aa250-400, DY550) - LS-C143509 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KDM3A / JMJD1A Antibody - LS-C153185 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-KDM3A / JMJD1A Antibody (aa1181) - LS-C190215 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-KDM3A / JMJD1A Antibody (aa983-1017, AP) - LS-C215317 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KDM3A / JMJD1A Antibody (aa983-1017, FITC) - LS-C215320 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KDM3A / JMJD1A Antibody (aa983-1017, APC) - LS-C215319 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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