Anti-KDM4B / JMJD2B antibody
| Name | Anti-KDM4B / JMJD2B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab27531 |
| Prices | $388.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human JMJD2B |
| Description | Rabbit Polyclonal |
| Gene | KDM4B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-KDM4B / JMJD2B antibody (ab27531) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab27531 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27531 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
An epigenetic role for PRL-3 as a regulator of H3K9 methylation in colorectal - An epigenetic role for PRL-3 as a regulator of H3K9 methylation in colorectal
Liu Y, Zheng P, Liu Y, Ji T, Liu X, Yao S, Cheng X, Li Y, Chen L, Xiao Z, Zhou J, Li J. Gut. 2013 Apr;62(4):571-81.
The histone demethylases JMJD1A and JMJD2B are transcriptional targets of - The histone demethylases JMJD1A and JMJD2B are transcriptional targets of
Beyer S, Kristensen MM, Jensen KS, Johansen JV, Staller P. J Biol Chem. 2008 Dec 26;283(52):36542-52.