![Overlay histogram showing HeLa cells stained with ab123900 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab123900, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/12237_ab123900-14-ab123900FC.jpg)
Overlay histogram showing HeLa cells stained with ab123900 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab123900, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-KDM4C / GASC1 / JMJD2C antibody [5B9] (ab123900) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY KDM4C / GASC1 / JMJD2C cDNALysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/12238_KDM4C-GASC1-JMJD2C-Primary-antibodies-ab123900-1.jpg)
All lanes : Anti-KDM4C / GASC1 / JMJD2C antibody [5B9] (ab123900) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY KDM4C / GASC1 / JMJD2C cDNALysates/proteins at 5 µg per lane.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human colon tissue by Immunohistochemistry.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human ovary tissue by Immunohistochemistry.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human prostate tissue by Immunohistochemistry.
ab123900, at 1/50 dilution, staining KDM4C / GASC1 / JMJD2C in paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
ab123900, at 1/100 dilution, staining KDM4C / GASC1 / JMJD2C in COS7 cells transiently transfected with pCMV6-ENTRY KDM4C / GASC1 / JMJD2C by Immunofluorescence.
![All lanes : Anti-KDM4C / GASC1 / JMJD2C antibody [5B9] (ab123900) at 1/1000 dilutionLane 1 : Neonatal rat cardiac myocytesLane 2 : Neonatal rat cardiac myocytes infected with adenovirus expressing Human KDM4C / GASC1 / JMJD2C (MOI 8)Lane 3 : Neonatal rat cardiac myocytes infected with adenovirus expressing Human KDM4C / GASC1 / JMJD2C (MOI 50)Lysates/proteins at 50 µg per lane.SecondaryPeroxidase conjugated anti-Mouse polyclonal IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/12250_KDM4C-GASC1-JMJD2C-Primary-antibodies-ab123900-28.jpg)
All lanes : Anti-KDM4C / GASC1 / JMJD2C antibody [5B9] (ab123900) at 1/1000 dilutionLane 1 : Neonatal rat cardiac myocytesLane 2 : Neonatal rat cardiac myocytes infected with adenovirus expressing Human KDM4C / GASC1 / JMJD2C (MOI 8)Lane 3 : Neonatal rat cardiac myocytes infected with adenovirus expressing Human KDM4C / GASC1 / JMJD2C (MOI 50)Lysates/proteins at 50 µg per lane.SecondaryPeroxidase conjugated anti-Mouse polyclonal IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.