Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade
| Name | Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab34718 |
| Prices | $392.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Human, Pig |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1500 to the C-terminus of Human Jarid1C/ SMCX |
| Description | Rabbit Polyclonal |
| Gene | KDM5C |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718) at 1/250 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902) at 20 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab34718 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34718 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Identification of telomere-associated molecules by engineered DNA-binding - Identification of telomere-associated molecules by engineered DNA-binding
Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H. Sci Rep. 2013 Nov 8;3:3171.
Histone deacetylase inhibitors stimulate histone H3 lysine 4 methylation in part - Histone deacetylase inhibitors stimulate histone H3 lysine 4 methylation in part
Huang PH, Chen CH, Chou CC, Sargeant AM, Kulp SK, Teng CM, Byrd JC, Chen CS. Mol Pharmacol. 2011 Jan;79(1):197-206.
C-terminal binding protein maintains mitochondrial activities. - C-terminal binding protein maintains mitochondrial activities.
Kim JH, Youn HD. Cell Death Differ. 2009 Apr;16(4):584-92.