All lanes : Anti-KDM5C / Jarid1C / SMCX antibody - N-terminal (ab190181) at 1/2000 dilutionLane 1 : Human U2OS cell lysateLane 2 : Human U2OS cell lysate transfected with KDM5C / Jarid1C / SMCX siRNALane 3 : Mouse embryo fibroblasts (MEFs) cell lysateLane 4 : Rat INS1 cell lysateLane 5 : Chicken DT40 cell lysateLysates/proteins at 30 µg per lane.
All lanes : Anti-KDM5C / Jarid1C / SMCX antibody - N-terminal (ab190181) at 1/2000 dilutionLane 1 : Xenopus laevis crude egg extractsLane 2 : U2OS whole cell lysateLysates/proteins at 50 µg per lane.
Western blot analysis using ab190181 of immunoprecipitated U2OS cells extracts:Lane 1: Input.Lane 2: Immunoprecipitation with control serum.Lane 3: Immunoprecipitation with ab190181 (5 µL)
Immunofluorescence analysis of U2OS cells labeling KDMC / Jarid1C / SMCX (green) using ab190181 at a 1/2000 dilution. Nuclei were stained with DAPI (blue).