Anti-KDM6A / UTX antibody
| Name | Anti-KDM6A / UTX antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab36938 |
| Prices | $375.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Species Reactivities | Mouse, Human, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human UTX |
| Description | Rabbit Polyclonal |
| Gene | KDM6A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-KDM6A / UTX antibody (ab36938) at 1 µg/mlLane 1 : Whole NIH3T3 cell lysate (no shRNA)Lane 2 : Whole NIH3T3 cell lysate (Control shRNA)Lane 3 : Whole NIH3T3 cell lysate (Utx shRNA)Lysates/proteins at 20 µg per lane.SecondaryHRP-conjugated Goat anti-Rabbit polyclonaldeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-KDM6A / UTX antibody (ab36938) at 1 µg/mlLane 1 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)Lane 2 : Testis (Mouse) Tissue LysateLane 3 : Liver (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG -H&L- Pre Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab36938 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab36938, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa cells fixed in 4% PFA at 1ug/ml and HeLa and MCF7 100% methanol fixed cells at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Product References
Stage-dependent and locus-specific role of histone demethylase Jumonji D3 (JMJD3) - Stage-dependent and locus-specific role of histone demethylase Jumonji D3 (JMJD3)
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Contrasting roles of histone 3 lysine 27 demethylases in acute lymphoblastic - Contrasting roles of histone 3 lysine 27 demethylases in acute lymphoblastic
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Ezh1 and Ezh2 differentially regulate PSD-95 gene transcription in developing - Ezh1 and Ezh2 differentially regulate PSD-95 gene transcription in developing
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Epigenetic changes in renal genes dysregulated in mouse and rat models of type 1 - Epigenetic changes in renal genes dysregulated in mouse and rat models of type 1
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H3K27 demethylase, JMJD3, regulates fragmentation of spermatogonial cysts. - H3K27 demethylase, JMJD3, regulates fragmentation of spermatogonial cysts.
Iwamori N, Iwamori T, Matzuk MM. PLoS One. 2013 Aug 15;8(8):e72689.
KSHV PAN RNA associates with demethylases UTX and JMJD3 to activate lytic - KSHV PAN RNA associates with demethylases UTX and JMJD3 to activate lytic
Rossetto CC, Pari G. PLoS Pathog. 2012;8(5):e1002680.
Bromodomain protein 7 interacts with PRMT5 and PRC2, and is involved in - Bromodomain protein 7 interacts with PRMT5 and PRC2, and is involved in
Tae S, Karkhanis V, Velasco K, Yaneva M, Erdjument-Bromage H, Tempst P, Sif S. Nucleic Acids Res. 2011 Jul;39(13):5424-38.
Evidence for alteration of EZH2, BMI1, and KDM6A and epigenetic reprogramming in - Evidence for alteration of EZH2, BMI1, and KDM6A and epigenetic reprogramming in
Hyland PL, McDade SS, McCloskey R, Dickson GJ, Arthur K, McCance DJ, Patel D. J Virol. 2011 Nov;85(21):10999-1006.
Carcinoma in situ testis displays permissive chromatin modifications similar to - Carcinoma in situ testis displays permissive chromatin modifications similar to
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Folic acid remodels chromatin on Hes1 and Neurog2 promoters during caudal neural - Folic acid remodels chromatin on Hes1 and Neurog2 promoters during caudal neural
Ichi S, Costa FF, Bischof JM, Nakazaki H, Shen YW, Boshnjaku V, Sharma S, Mania-Farnell B, McLone DG, Tomita T, Soares MB, Mayanil CS. J Biol Chem. 2010 Nov 19;285(47):36922-32.