ChIP analysis of recruitment of CBP, p300, PCA and BRG1 to the IL-2α (CD25) promoter
in vivo. Antibodies tested include CBP (A-22): sc-369, p300 (N-15): sc-584, PCAF (H-369): sc-8999, and Brg-1 (H-88): sc-10768. CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag
-/- thymocytes or Rag
-/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H.,
et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press.