SMARCA4

Gene Symbol SMARCA4
Entrez Gene 6597
Alt Symbol BAF190, BAF190A, BRG1, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b
Species Human
Gene Type protein-coding
Description SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Other Description ATP-dependent helicase SMARCA4|BRG1-associated factor 190A|BRM/SWI2-related gene 1|SNF2-beta|SNF2-like 4|brahma protein-like 1|global transcription activator homologous sequence|homeotic gene regulator|mitotic growth and transcription activator|nuclear protein GRB1|protein BRG-1|protein brahma homolog 1|sucrose nonfermenting-like 4|transcription activator BRG1
Swissprots B1A8Z5 B1A8Z4 B1A8Z7 O95052 P51532 E9PBR8 B1A8Z6 Q9HBD3
Accessions AAC97986 AAC97987 AAG24789 AAG24790 EAW84162 EAW84163 EAW84164 EAW84165 EAW84166 EAW84167 P51532 AB209313 BAD92550 AI695800 AK026573 AK055168 BAG51479 AK097105 AK290033 BAF82722 AK299761 BAG61650 AK303352 BAG64413 AK308763 BC007302 BC035916 BC136644 AAI36645 BC139917 AAI39918 BC139924 AAI39925 BC150298 AAI50299 BC151849 AAI51850 BG396607 BQ009907 CT005139 D26156 BAA05143 EU430756 ACA09750 EU430757 ACA09751 EU430758 ACA09752 EU430759 ACA09753 U29175 AAB40977 XM_005260028 XP_005260085 XM_005260030 XP_005260087 XM_005260031 XP_005260088 XM_005260032 XP_005260089 XM_005260033 XP_005260090 XM_005260034 XP_005260091 XM_005260035 XP_005260092 XM_006722845 XP_006722908 XM_006722846 XP_006722909 XM_006722847 XP_006722910 XM_011528198 XP_011526500 NM_001128844 NP_001122316 NM_001128845 NP_001122317 NM_001128846 NP_001122318 NM_001128847 NP_001122319 NM_001128848 NP_001122320 NM_001128849 NP_001122321 NM_003072 NP_003063
Function Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chroma
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00549, ECO:0000269|PubMed:20418909}.
Tissue Specificity Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). {ECO:0000269|PubMed:20418909}.

Brg1 (P680) Antibody - 3514 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines using Brg1 (P680) Antibody.

Brg1 (A52) Antibody - 3508 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines using Brg1 (A52) Antibody.

Brg-1 (H-10) - sc-374197 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Brg-1 (H-10): sc-374197. Western blot analysis of Brg-1 expression in K-562 nuclear extract.

Brg-1 (G-7) - sc-17796 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Brg-1 (G-7): sc-17796. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.

Brg-1 (P-18) - sc-12520 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Brg-1 (P-18): sc-12520. Western blot analysis of Brg-1 expression in K-562 (A) and HeLa (B) nuclear extracts.

Brg-1 (N-15) - sc-8749 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Brg-1 (N-15): sc-8749. Western blot analysis of Brg-1 expression in K-562 (A) and HeLa (B) nuclear extracts.

Brg-1 (H-88) - sc-10768 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Brg-1 (H-88): sc-10768. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing nuclear staining.

Anti-BRG1 antibody - ab4081 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BRG1 antibody (ab4081) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-BRG1 antibody - ab70558 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse plasmacytoma tissue labelling BRG1 with ab70558 at 1/1000 (0.2µg/ml). Detection: DAB.

Anti-BRG1 antibody - ab118558 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab118558 at 7.5&microg/ml staining BRG1 in formalin fixed paraffin embedded Human prostate tissue by immunohistochemistry.

Anti-BRG1 antibody - ab91594 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human BRG1 by Immunohistochemistry on Formalin Fixed Paraffin Embedded section of human nonsmall cell lung cancer using 1/250 ab91594. Detection: DAB staining.

Anti-BRG1 antibody - ab135079 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-BRG1 antibody (ab135079) at 1/1000 dilutionLane 1 : HeLa nuclear extractLane 2 : HepG2 total cell extract

Anti-BRG1 antibody [BRG-01] - ab84945 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone BRG-01

Anti-BRG1 antibody [EPNCIR111A] - ab110641 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR111A
All lanes : Anti-BRG1 antibody [EPNCIR111A] (ab110641) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : MOLT4 cell lysateLane 4 : NIH3T3 cell lysateLane 5 : PC12 cell lysateLysates/proteins at 10 µg per lane.

Anti-BRG1 antibody [EPNCIR111A] (Alexa Fluor® 647) - ab196535 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR111A
ab196535 staining BRG1 in SW480 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196535 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-BRG1 antibody [EPNCIR111A] (HRP) - ab196315 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR111A
All lanes : Anti-BRG1 antibody [EPNCIR111A] (HRP) (ab196315) at 1/5000 dilutionLane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-BRG1 antibody [EPNCIR111B] - ab133257 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR111B
All lanes : Anti-BRG1 antibody [EPNCIR111B] (ab133257) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : Molt 4 cell lysateLane 4 : PC12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody. at 1/2000 dilution

Anti-BRG1 antibody [EPR3912] - ab108318 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3912
All lanes : Anti-BRG1 antibody [EPR3912] (ab108318) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : Molt-4 cell lysateLysates/proteins at 10 µg per lane.

Anti-BRG1 antibody [EPR3913] - ab92496 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3913
All lanes : Anti-BRG1 antibody [EPR3913] (ab92496) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : MOLT4 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-BRG1 antibody [SMAR9G1F9] - ab51509 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone SMAR9G1F9

Anti-BRG1 antibody - C-terminal - ab182790 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-BRG1 antibody - C-terminal (ab182790) at 1/700 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 50 µg per lane.SecondaryGoat anti Rabbit IgG at 1/10000 dilution

Anti-SMARCA4 / BRG1 Antibody (clone EPR3912) - LS-C137725 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3912
IHC of paraffin-embedded human colon tissue using anti-BRG1 antibody

Anti-SMARCA4 / BRG1 Antibody (aa2-14) IHC-plus™ - LS-B5107 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-SMARCA4 / BRG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5107 concentration 3.75 ug/ml.

Anti-SMARCA4 / BRG1 Antibody (aa50-100) - LS-C290525 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Goat
Clonality Polyclonal
Detection of Mouse BRG1 by Immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BRG1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-SMARCA4 / BRG1 Antibody (C-Terminus) IHC-plus™ - LS-B12057 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
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