Anti-KHSRP antibody

• Supplier: Abcam
• Catalog: ab56438
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Applications: IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Recombinant fragment: VPDGMVGLII GRGGEQINKI QQDSGCKVQI SPDSGGLPER SVSLTGAPES VQKAKMMLDD IVSRGRGGPP GQFHDNANGG QNGTVQEIM, corresponding to amino acids 151-240 of Human KHSRP Run BLAST with Run BLAST with
• Description: Mouse Monoclonal
• Gene: KHSRP
• Conjugate: Unconjugated

Product images

KHSRP antibody (ab56438) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human liver.

Western blot against tagged recombinant protein immunogen using ab56438 KHSRP antibody at 1ug/ml.

ICC/IF image of ab56438 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56438, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Novel proteomic strategy reveal combined alpha1 antitrypsin and cathepsin D as - Novel proteomic strategy reveal combined alpha1 antitrypsin and cathepsin D as

Xie LQ, Zhao C, Cai SJ, Xu Y, Huang LY, Bian JS, Shen CP, Lu HJ, Yang PY. J Proteome Res. 2010 Sep 3;9(9):4701-9.

Proteomic analysis of cells in the early stages of herpes simplex virus type-1 - Proteomic analysis of cells in the early stages of herpes simplex virus type-1

Antrobus R, Grant K, Gangadharan B, Chittenden D, Everett RD, Zitzmann N, Boutell C. Proteomics. 2009 Aug;9(15):3913-27.