Anti-Ki67 antibody
| Name | Anti-Ki67 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab833 |
| Prices | $394.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ICC/IF IHC-P IHC-F |
| Species Reactivities | Human, Monkey |
| Antigen | Synthetic peptide corresponding to Human Ki67 |
| Description | Rabbit Polyclonal |
| Gene | MKI67 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab833 - immunohistochemistry Formalin fixed paraffin embedded human tonsil stained with Ki-67, using ABC and AEC chromogen.
This picture was kindly supplied as part of the review submitted by Dr Robert O'Donogue. Tissue sections from mouse lung were stained with ab833 at a dilution of 1/50.
HT-29 xenograft tumor in mouse 20x pretreatment w/ citrate pH 6.0 using anti-Ki67 antibody ab833 at 5 ug/mL for 30 min at RT.
ab833 at 1/50 dilution staining pig skeletal myeloblast cells by ICC/IF. The cells were fixed with 2% paraformaldehyde for 15 min and permealized with Triton-X100 (0.1%) for 10 min prior to incubation with the primary antibody overnight at 4° C. The Image shows DAPI (blue-nuclear stain, upper left panel), Ki-67 (Green, upper right panel, shows nuclear localization in actively dividing cells), Skeletal myoblast DIC (phase) image (lower left panel) and overlay image (lower right panel). Because Ki67 antigen has a short half-life and is expressed only during the G1, S, G2, and early mitosis and not during the G0 (resting) phase Ki67was used as a marker of actively proliferating skeletal muscle satellite stem cells. See Abreview
Immunohistochemical analysis of Human skin tissue, staining Ki67 (red) with ab833. Antigen retrieval was performed by heat mediation in a EDTA/Tris buffer (pH 8.0). Sections were blocked with 10% NGS for 1 hour at room temperature, followed by incubation with primary antibody (1/25). An AlexFluor®546 anti-rabbit IgG (1/500) was used as the secondary antibody.
Product References
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